EMD-21246

Single-particle
4.25 Å
EMD-21246 Deposition: 29/01/2020
Map released: 24/06/2020
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21246

BG505 SOSIP.v4.1 in complex with rhesus macaque Fab RM20F

EMD-21246

Single-particle
4.25 Å
EMD-21246 Deposition: 29/01/2020
Map released: 24/06/2020
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Human immunodeficiency virus 1, Macaca mulatta
Sample: BG505 SOSIP.v4.1 in complex with rhesus macaque Fab RM20F
Fitted models: 6vn0 (Avg. Q-score: 0.345)

Deposition Authors: Cottrell CA , Shin M
Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates.
PUBMED: 32866207
DOI: doi:10.1371/journal.ppat.1008753
ISSN: 1553-7374
Abstract:
The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.