EMD-23785

Single-particle
3.2 Å
EMD-23785 Deposition: 06/04/2021
Map released: 01/09/2021
Last modified: 20/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-23785

80S rabbit ribosome stalled with benzamide-CHX

EMD-23785

Single-particle
3.2 Å
EMD-23785 Deposition: 06/04/2021
Map released: 01/09/2021
Last modified: 20/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Oryctolagus cuniculus, synthetic construct
Sample: 80S rabbit ribosome stalled with benzamide-CHX
Fitted models: 7mdz (Avg. Q-score: 0.502)

Deposition Authors: Koga Y , Hoang EM
Discovery of C13-Aminobenzoyl Cycloheximide Derivatives that Potently Inhibit Translation Elongation.
Koga Y , Hoang EM , Park Y , Keszei AFA , Murray J , Shao S , Liau BB
(2021) J Am Chem Soc , 143 , 13473 - 13477
PUBMED: 34403584
DOI: doi:10.1021/jacs.1c05146
ISSN: 1520-5126
ASTM: JACSAT
Abstract:
Employed for over half a century to study protein synthesis, cycloheximide (CHX, 1) is a small molecule natural product that reversibly inhibits translation elongation. More recently, CHX has been applied to ribosome profiling, a method for mapping ribosome positions on mRNA genome-wide. Despite CHX's extensive use, CHX treatment often results in incomplete translation inhibition due to its rapid reversibility, prompting the need for improved reagents. Here, we report the concise synthesis of C13-amide-functionalized CHX derivatives with increased potencies toward protein synthesis inhibition. Cryogenic electron microscopy (cryo-EM) revealed that C13-aminobenzoyl CHX (8) occupies the same site as CHX, competing with the 3' end of E-site tRNA. We demonstrate that 8 is superior to CHX for ribosome profiling experiments, enabling more effective capture of ribosome conformations through sustained stabilization of polysomes. Our studies identify powerful chemical reagents to study protein synthesis and reveal the molecular basis of their enhanced potency.