EMD-24196
SARS-CoV-2 spike (6P) in complex with C93D9 Fab
EMD-24196
Single-particle7.1 Å
Deposition: 07/06/2021Map released: 04/08/2021
Last modified: 04/08/2021
Sample Organism:
Severe acute respiratory syndrome coronavirus 2
Sample: SARS-CoV-2 Spike (2P) in complex with C93D9 Fab
Deposition Authors: Windsor IW, Tong P, Gautam AK, Wesemann DR, Harrison SC
Sample: SARS-CoV-2 Spike (2P) in complex with C93D9 Fab
Deposition Authors: Windsor IW, Tong P, Gautam AK, Wesemann DR, Harrison SC
Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.
Tong P,
Gautam A,
Windsor I,
Travers M,
Chen Y,
Garcia N,
Whiteman NB,
McKay LGA,
Lelis FJN,
Habibi S,
Cai Y,
Rennick LJ,
Duprex WP,
McCarthy KR,
Lavine CL,
Zuo T,
Lin J,
Zuiani A,
Feldman J,
MacDonald EA,
Hauser BM,
Griffths A,
Seaman MS,
Schmidt AG,
Chen B,
Neuberg D,
Bajic G,
Harrison SC,
Wesemann DR
(2021) bioRxiv
(2021) bioRxiv
Abstract:
Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.
Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.