EMD-25005
Spike proteins from OC43, HKU1, MERS, and SARS CoVs complexed with polyclonal Fab from donor 1383
EMD-25005
Single-particle20.0 Å
Deposition: 26/09/2021Map released: 04/05/2022
Last modified: 18/05/2022
Sample Organism:
Human coronavirus OC43
Sample: Spike proteins from OC43, HKU1, MERS, and SARS CoVs complexed with polyclonal Fab from donor 1383
Deposition Authors: Ward AB
,
Bangaru S ,
Sewall LM,
Jackson AM
Sample: Spike proteins from OC43, HKU1, MERS, and SARS CoVs complexed with polyclonal Fab from donor 1383
Deposition Authors: Ward AB
,
Bangaru S ,
Sewall LM,
Jackson AM
Structural mapping of antibody landscapes to human betacoronavirus spike proteins.
Bangaru S ,
Antanasijevic A ,
Kose N,
Sewall LM,
Jackson AM ,
Suryadevara N ,
Zhan X ,
Torres JL ,
Copps J ,
de la Pena AT,
Crowe Jr JE ,
Ward AB
(2022) Sci Adv , 8 , eabn2911 - eabn2911
,
Antanasijevic A ,
Kose N,
Sewall LM,
Jackson AM ,
Suryadevara N ,
Zhan X ,
Torres JL ,
Copps J ,
de la Pena AT,
Crowe Jr JE ,
Ward AB
(2022) Sci Adv , 8 , eabn2911 - eabn2911
Abstract:
Preexisting immunity against seasonal coronaviruses (CoVs) represents an important variable in predicting antibody responses and disease severity to severe acute respiratory syndrome CoV-2 (SARS-CoV-2) infections. We used electron microscopy-based polyclonal epitope mapping (EMPEM) to characterize the antibody specificities against β-CoV spike proteins in prepandemic (PP) sera or SARS-CoV-2 convalescent (SC) sera. We observed that most PP sera had antibodies specific to seasonal human CoVs (HCoVs) OC43 and HKU1 spike proteins while the SC sera showed reactivity across all human β-CoVs. Detailed molecular mapping of spike-antibody complexes revealed epitopes that were differentially targeted by preexisting antibodies and SC serum antibodies. Our studies provide an antigenic landscape to β-HCoV spikes in the general population serving as a basis for cross-reactive epitope analyses in SARS-CoV-2-infected individuals.
Preexisting immunity against seasonal coronaviruses (CoVs) represents an important variable in predicting antibody responses and disease severity to severe acute respiratory syndrome CoV-2 (SARS-CoV-2) infections. We used electron microscopy-based polyclonal epitope mapping (EMPEM) to characterize the antibody specificities against β-CoV spike proteins in prepandemic (PP) sera or SARS-CoV-2 convalescent (SC) sera. We observed that most PP sera had antibodies specific to seasonal human CoVs (HCoVs) OC43 and HKU1 spike proteins while the SC sera showed reactivity across all human β-CoVs. Detailed molecular mapping of spike-antibody complexes revealed epitopes that were differentially targeted by preexisting antibodies and SC serum antibodies. Our studies provide an antigenic landscape to β-HCoV spikes in the general population serving as a basis for cross-reactive epitope analyses in SARS-CoV-2-infected individuals.