EMD-26525

Single-particle
25.0 Å
EMD-26525 Deposition: 28/03/2022
Map released: 08/06/2022
Last modified: 17/01/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-26525

SARS-CoV-2 6P Mut7 in complex with K398.16 Fab (3 bound)

EMD-26525

Single-particle
25.0 Å
EMD-26525 Deposition: 28/03/2022
Map released: 08/06/2022
Last modified: 17/01/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Severe acute respiratory syndrome coronavirus 2, Macaca mulatta
Sample: SARS-CoV-2 6P Mut7 in complex with K398.16 Fab (3 bound)

Deposition Authors: Lee WH , Torres JL , Ward AB
Broadly neutralizing antibodies to SARS-related viruses can be readily induced in rhesus macaques.
PUBMED: 35947674
DOI: doi:10.1126/scitranslmed.abl9605
ISSN: 1946-6242
Abstract:
To prepare for future coronavirus (CoV) pandemics, it is desirable to generate vaccines capable of eliciting broadly neutralizing antibody responses to CoVs. Here, we show that immunization of macaques with SARS-CoV-2 spike (S) protein with a two-shot protocol generated potent serum receptor binding domain cross-neutralizing antibody responses to both SARS-CoV-2 and SARS-CoV-1. Furthermore, responses were equally effective against most SARS-CoV-2 variants of concern (VOCs) and some were highly effective against Omicron. This result contrasts with human infection or many two-shot vaccination protocols where responses were typically more SARS-CoV-2 specific and where VOCs were less well neutralized. Structural studies showed that cloned macaque neutralizing antibodies, particularly using a given heavy chain germline gene, recognized a relatively conserved region proximal to the angiotensin converting enzyme 2 receptor binding site (RBS), whereas many frequently elicited human neutralizing antibodies targeted more variable epitopes overlapping the RBS. B cell repertoire differences between humans and macaques appeared to influence the vaccine response. The macaque neutralizing antibodies identified a pan-SARS-related virus epitope region less well targeted by human antibodies that could be exploited in rational vaccine design.