EMD-26788
CPV Total-Fab Polyclonal A Site Fab
EMD-26788
Single-particle3.6 Å
Deposition: 27/04/2022
Map released: 04/10/2023
Last modified: 16/10/2024
Sample Organism:
Canis lupus familiaris
Sample: Canine parvovirus in complex with antibody fragment
Fitted models: 7uts (Avg. Q-score: 0.327)
Deposition Authors: Hartmann SR , Hafenstein SL , Charnesky AJ
Sample: Canine parvovirus in complex with antibody fragment
Fitted models: 7uts (Avg. Q-score: 0.327)
Deposition Authors: Hartmann SR , Hafenstein SL , Charnesky AJ
Cryo EM structures map a post vaccination polyclonal antibody response to canine parvovirus.
Hartmann SR ,
Charnesky AJ,
Fruh SP,
Lopez-Astacio RA,
Weichert WS,
DiNunno N,
Cho SH,
Bator CM,
Parrish CR ,
Hafenstein SL
(2023) Commun Biol , 6 , 955 - 955
(2023) Commun Biol , 6 , 955 - 955
Abstract:
Canine parvovirus (CPV) is an important pathogen that emerged by cross-species transmission to cause severe disease in dogs. To understand the host immune response to vaccination, sera from dogs immunized with parvovirus are obtained, the polyclonal antibodies are purified and used to solve the high resolution cryo EM structures of the polyclonal Fab-virus complexes. We use a custom software, Icosahedral Subparticle Extraction and Correlated Classification (ISECC) to perform subparticle analysis and reconstruct polyclonal Fab-virus complexes from two different dogs eight and twelve weeks post vaccination. In the resulting polyclonal Fab-virus complexes there are a total of five distinct Fabs identified. In both cases, any of the five antibodies identified would interfere with receptor binding. This polyclonal mapping approach identifies a specific, limited immune response to the live vaccine virus and allows us to investigate the binding of multiple different antibodies or ligands to virus capsids.
Canine parvovirus (CPV) is an important pathogen that emerged by cross-species transmission to cause severe disease in dogs. To understand the host immune response to vaccination, sera from dogs immunized with parvovirus are obtained, the polyclonal antibodies are purified and used to solve the high resolution cryo EM structures of the polyclonal Fab-virus complexes. We use a custom software, Icosahedral Subparticle Extraction and Correlated Classification (ISECC) to perform subparticle analysis and reconstruct polyclonal Fab-virus complexes from two different dogs eight and twelve weeks post vaccination. In the resulting polyclonal Fab-virus complexes there are a total of five distinct Fabs identified. In both cases, any of the five antibodies identified would interfere with receptor binding. This polyclonal mapping approach identifies a specific, limited immune response to the live vaccine virus and allows us to investigate the binding of multiple different antibodies or ligands to virus capsids.