EMD-28830

Single-particle
3.28 Å
EMD-28830 Deposition: 09/11/2022
Map released: 15/11/2023
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-28830

ELIC with Propylamine in saposin nanodiscs with 2:1:1 POPC:POPE:POPG

EMD-28830

Single-particle
3.28 Å
EMD-28830 Deposition: 09/11/2022
Map released: 15/11/2023
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Dickeya dadantii
Sample: ELIC with Propylamine in saposin nanodiscs with 2:1:1 POPC:POPE:POPG
Fitted models: 8f33 (Avg. Q-score: 0.496)

Deposition Authors: Dalal V , Arcario MJ , Petroff II JT , Deitzen NM, Tan BK, Brannigan G , Cheng WWL
Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel.
PUBMED: 38167383
DOI: doi:10.1038/s41467-023-44366-w
ISSN: 2041-1723
Abstract:
Lipid nanodiscs have become a standard tool for studying membrane proteins, including using single particle cryo-electron microscopy (cryo-EM). We find that reconstituting the pentameric ligand-gated ion channel (pLGIC), Erwinia ligand-gated ion channel (ELIC), in different nanodiscs produces distinct structures by cryo-EM. The effect of the nanodisc on ELIC structure extends to the extracellular domain and agonist binding site. Additionally, molecular dynamic simulations indicate that nanodiscs of different size impact ELIC structure and that the nanodisc scaffold directly interacts with ELIC. These findings suggest that the nanodisc plays a crucial role in determining the structure of pLGICs, and that reconstitution of ion channels in larger nanodiscs may better approximate a lipid membrane environment.