EMD-30875

Single-particle
2.89 Å
EMD-30875 Deposition: 14/01/2021
Map released: 31/03/2021
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-30875

Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)

EMD-30875

Single-particle
2.89 Å
EMD-30875 Deposition: 14/01/2021
Map released: 31/03/2021
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: the activated human minor spliceosome (human minor Bact complex)
Fitted models: 7dvq (Avg. Q-score: 0.432)

Deposition Authors: Bai R , Wan R
Structure of the activated human minor spliceosome.
Bai R , Wan R , Wang L , Xu K , Zhang Q , Lei J , Shi Y
(2021) Science , 371
PUBMED: 33509932
DOI: doi:10.1126/science.abg0879
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
The minor spliceosome mediates splicing of the rare but essential U12-type precursor messenger RNA. Here, we report the atomic features of the activated human minor spliceosome determined by cryo-electron microscopy at 2.9-angstrom resolution. The 5' splice site and branch point sequence of the U12-type intron are recognized by the U6atac and U12 small nuclear RNAs (snRNAs), respectively. Five newly identified proteins stabilize the conformation of the catalytic center: The zinc finger protein SCNM1 functionally mimics the SF3a complex of the major spliceosome, the RBM48-ARMC7 complex binds the γ-monomethyl phosphate cap at the 5' end of U6atac snRNA, the U-box protein PPIL2 coordinates loop I of U5 snRNA and stabilizes U5 small nuclear ribonucleoprotein (snRNP), and CRIPT stabilizes U12 snRNP. Our study provides a framework for the mechanistic understanding of the function of the human minor spliceosome.