EMD-31075

Single-particle
4.5 Å
EMD-31075 Deposition: 17/03/2021
Map released: 05/05/2021
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-31075

p53-bound TFIID-based core PIC on HDM2 promoter

EMD-31075

Single-particle
4.5 Å
EMD-31075 Deposition: 17/03/2021
Map released: 05/05/2021
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens, Sus scrofa
Sample: p53-bound TFIID-based core PIC on HDM2 promoter
Fitted models: 7edx (Avg. Q-score: 0.13)

Deposition Authors: Chen X , Qi Y
Structural insights into preinitiation complex assembly on core promoters.
Chen X , Qi Y , Wu Z, Wang X , Li J , Zhao D , Hou H , Li Y , Yu Z , Liu W , Wang M, Ren Y, Li Z , Yang H , Xu Y
(2021) Science , 372
PUBMED: 33795473
DOI: doi:10.1126/science.aba8490
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.