EMD-32465

Single-particle
4.33 Å
EMD-32465 Deposition: 26/12/2021
Map released: 16/03/2022
Last modified: 26/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-32465

Subcomplexes A and E in NDH complex from Arabidopsis

EMD-32465

Single-particle
4.33 Å
EMD-32465 Deposition: 26/12/2021
Map released: 16/03/2022
Last modified: 26/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Arabidopsis thaliana
Sample: Subcomplex A and E in NDH complex from Arabidopsis
Fitted models: 7wfg (Avg. Q-score: 0.232)

Deposition Authors: Pan XW, Li M
Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana.
Su X, Cao D, Pan X, Shi L, Liu Z, Dall'Osto L , Bassi R, Zhang X, Li M
(2022) Mol Plant , 15 , 454 - 467
PUBMED: 35123031
DOI: doi:10.1016/j.molp.2022.01.020
ISSN: 1752-9867
Abstract:
Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.9 Å resolution structure of the Arabidopsis thaliana NDH-PSI (AtNDH-PSI) supercomplex. We constructed structural models for 26 AtNDH subunits, among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex. In the supercomplex, one NDH can bind up to two PSI-light-harvesting complex I (PSI-LHCI) complexes at both sides of its membrane arm. Two minor LHCIs, Lhca5 and Lhca6, each present in one PSI-LHCI, interact with NDH and contribute to supercomplex formation and stabilization. Collectively, our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.