EMD-33021

Single-particle
3.15 Å
EMD-33021 Deposition: 07/03/2022
Map released: 24/05/2023
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
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EMD-33021

Cryo-EM structure of the human TRPC5 ion channel in lipid nanodiscs, class1

EMD-33021

Single-particle
3.15 Å
EMD-33021 Deposition: 07/03/2022
Map released: 24/05/2023
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: Transient receptor potential
Fitted models: 7x6c (Avg. Q-score: 0.518)

Deposition Authors: Won J , Jeong H , Lee HH
Molecular architecture of the G alpha i -bound TRPC5 ion channel.
Won J , Kim J , Jeong H , Kim J , Feng S , Jeong B, Kwak M, Ko J , Im W , So I , Lee HH
(2023) Nat Commun , 14 , 2550 - 2550
PUBMED: 37137991
DOI: doi:10.1038/s41467-023-38281-3
ISSN: 2041-1723
Abstract:
G-protein coupled receptors (GPCRs) and ion channels serve as key molecular switches through which extracellular stimuli are transformed into intracellular effects, and it has long been postulated that ion channels are direct effector molecules of the alpha subunit of G-proteins (Gα). However, no complete structural evidence supporting the direct interaction between Gα and ion channels is available. Here, we present the cryo-electron microscopy structures of the human transient receptor potential canonical 5 (TRPC5)-Gαi3 complexes with a 4:4 stoichiometry in lipid nanodiscs. Remarkably, Gαi3 binds to the ankyrin repeat edge of TRPC5 ~ 50 Å away from the cell membrane. Electrophysiological analysis shows that Gαi3 increases the sensitivity of TRPC5 to phosphatidylinositol 4,5-bisphosphate (PIP2), thereby rendering TRPC5 more easily opened in the cell membrane, where the concentration of PIP2 is physiologically regulated. Our results demonstrate that ion channels are one of the direct effector molecules of Gα proteins triggered by GPCR activation-providing a structural framework for unraveling the crosstalk between two major classes of transmembrane proteins: GPCRs and ion channels.