EMD-33959

Single-particle
3.29 Å
EMD-33959 Deposition: 30/07/2022
Map released: 01/02/2023
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-33959

Cryo-EM structure of Cas7-11-crRNA-Csx29 ternary complex

EMD-33959

Single-particle
3.29 Å
EMD-33959 Deposition: 30/07/2022
Map released: 01/02/2023
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Desulfonema ishimotonii
Sample: Cas7-11-crRNA-Csx29 ternary complex
Fitted models: 7ynd (Avg. Q-score: 0.526)

Deposition Authors: Huo Y , Dong Q, Zhao H , Jiang T
Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.
Huo Y , Zhao H , Dong Q, Jiang T
(2023) Nat Microbiol , 8 , 522 - 532
PUBMED: 36702942
DOI: doi:10.1038/s41564-022-01316-4
ISSN: 2058-5276
Abstract:
The recently discovered type III-E CRISPR-Cas effector Cas7-11 shows promise when used as an RNA manipulation tool, but its structure and the mechanisms underlying its function remain unclear. Here we present four cryo-EM structures of Desulfonema ishimotonii Cas7-11-crRNA complex in pre-target and target RNA-bound states, and the cryo-EM structure of DiCas7-11-crRNA bound to its accessory protein DiCsx29. These data reveal structural elements for pre-crRNA processing, target RNA cleavage and regulation. Moreover, a 3' seed region of crRNA is involved in regulating RNA cleavage activity of DiCas7-11-crRNA-Csx29. Our analysis also shows that both the minimal mismatch of 4 nt to the 5' handle of crRNA and the minimal matching of the first 12 nt of the spacer by the target RNA are essential for triggering the protease activity of DiCas7-11-crRNA-Csx29 towards DiCsx30. Taken together, we propose that target RNA recognition and cleavage regulate and fine-tune the protease activity of DiCas7-11-crRNA-Csx29, thus preventing auto-immune responses.