EMD-34687
SARS-CoV-2 Spike trimer in complex with RmAb 9H1 Fab in the class 2 conformation
EMD-34687
Single-particle3.5 Å
Deposition: 08/11/2022Map released: 26/04/2023
Last modified: 06/11/2024
Sample Organism:
Severe acute respiratory syndrome coronavirus 2,
Oryctolagus cuniculus
Sample: SARS-CoV-2 WT spike glycoprotein complex with RmAb 9H1 Fabs
Fitted models: 8hec (Avg. Q-score: 0.358)
Deposition Authors: Guo H
,
Gao Y,
Lu Y ,
Yang H,
Ji X
Sample: SARS-CoV-2 WT spike glycoprotein complex with RmAb 9H1 Fabs
Fitted models: 8hec (Avg. Q-score: 0.358)
Deposition Authors: Guo H
,
Gao Y,
Lu Y ,
Yang H,
Ji X
Mechanism of an RBM-targeted rabbit monoclonal antibody 9H1 neutralizing SARS-CoV-2.
Chu X,
Ding X,
Yang Y,
Lu Y ,
Li T,
Gao Y,
Zheng L,
Xiao H,
Yang T,
Cheng H,
Huang H,
Liu Y,
Lou Y,
Wu C,
Chen Y ,
Yang H,
Ji X,
Guo H
(2023) Biochem Biophys Res Commun , 660 , 43 - 49
,
Li T,
Gao Y,
Zheng L,
Xiao H,
Yang T,
Cheng H,
Huang H,
Liu Y,
Lou Y,
Wu C,
Chen Y ,
Yang H,
Ji X,
Guo H
(2023) Biochem Biophys Res Commun , 660 , 43 - 49
Abstract:
The COVID-19 pandemic, caused by SARS-CoV-2, has led to over 750 million infections and 6.8 million deaths worldwide since late 2019. Due to the continuous evolution of SARS-CoV-2, many significant variants have emerged, creating ongoing challenges to the prevention and treatment of the pandemic. Therefore, the study of antibody responses against SARS-CoV-2 is essential for the development of vaccines and therapeutics. Here we perform single particle cryo-electron microscopy (cryo-EM) structure determination of a rabbit monoclonal antibody (RmAb) 9H1 in complex with the SARS-CoV-2 wild-type (WT) spike trimer. Our structural analysis shows that 9H1 interacts with the receptor-binding motif (RBM) region of the receptor-binding domain (RBD) on the spike protein and by directly competing with angiotensin-converting enzyme 2 (ACE2), it blocks the binding of the virus to the receptor and achieves neutralization. Our findings suggest that utilizing rabbit-derived mAbs provides valuable insights into the molecular interactions between neutralizing antibodies and spike proteins and may also facilitate the development of therapeutic antibodies and expand the antibody library.
The COVID-19 pandemic, caused by SARS-CoV-2, has led to over 750 million infections and 6.8 million deaths worldwide since late 2019. Due to the continuous evolution of SARS-CoV-2, many significant variants have emerged, creating ongoing challenges to the prevention and treatment of the pandemic. Therefore, the study of antibody responses against SARS-CoV-2 is essential for the development of vaccines and therapeutics. Here we perform single particle cryo-electron microscopy (cryo-EM) structure determination of a rabbit monoclonal antibody (RmAb) 9H1 in complex with the SARS-CoV-2 wild-type (WT) spike trimer. Our structural analysis shows that 9H1 interacts with the receptor-binding motif (RBM) region of the receptor-binding domain (RBD) on the spike protein and by directly competing with angiotensin-converting enzyme 2 (ACE2), it blocks the binding of the virus to the receptor and achieves neutralization. Our findings suggest that utilizing rabbit-derived mAbs provides valuable insights into the molecular interactions between neutralizing antibodies and spike proteins and may also facilitate the development of therapeutic antibodies and expand the antibody library.