EMD-35313

Single-particle
4.2 Å
EMD-35313 Deposition: 09/02/2023
Map released: 18/09/2024
Last modified: 04/12/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-35313

Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature

EMD-35313

Single-particle
4.2 Å
EMD-35313 Deposition: 09/02/2023
Map released: 18/09/2024
Last modified: 04/12/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Mus musculus
Sample: Respiratory Supercomplex CI:CIII2
Fitted models: 8iao (Avg. Q-score: 0.295)

Deposition Authors: Shin Y-C , Liao M
Structural basis of respiratory complex adaptation to cold temperatures.
PUBMED: 39395414
DOI: doi:10.1016/j.cell.2024.09.029
ISSN: 1097-4172
Abstract:
In response to cold, mammals activate brown fat for respiratory-dependent thermogenesis reliant on the electron transport chain. Yet, the structural basis of respiratory complex adaptation upon cold exposure remains elusive. Herein, we combined thermoregulatory physiology and cryoelectron microscopy (cryo-EM) to study endogenous respiratory supercomplexes from mice exposed to different temperatures. A cold-induced conformation of CI:III2 (termed type 2) supercomplex was identified with a ∼25° rotation of CIII2 around its inter-dimer axis, shortening inter-complex Q exchange space, and exhibiting catalytic states that favor electron transfer. Large-scale supercomplex simulations in mitochondrial membranes reveal how lipid-protein arrangements stabilize type 2 complexes to enhance catalytic activity. Together, our cryo-EM studies, multiscale simulations, and biochemical analyses unveil the thermoregulatory mechanisms and dynamics of increased respiratory capacity in brown fat at the structural and energetic level.