EMD-3580

Single-particle
7.6 Å
EMD-3580 Deposition: 25/01/2017
Map released: 26/04/2017
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-3580

E. coli expressome

EMD-3580

Single-particle
7.6 Å
EMD-3580 Deposition: 25/01/2017
Map released: 26/04/2017
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Escherichia coli K-12
Sample: in vitro reconstituted E. coli expressome consisting of transcribing RNA polymerase and translating ribosome
Fitted models: 5my1 (Avg. Q-score: 0.113)

Deposition Authors: Kohler R , Mooney RA
Architecture of a transcribing-translating expressome.
Kohler R , Mooney RA , Mills DJ , Landick R , Cramer P
(2017) Science , 356 , 194 - 197
PUBMED: 28408604
DOI: doi:10.1126/science.aal3059
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of Escherichia coli can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.