EMD-35846
Cellular components at INS-1E cell periphery under basal condition
EMD-35846
Tomography
Map released: 28/02/2024
Last modified: 28/02/2024
Sample Organism:
Rattus
Sample: INS-1E cell
Deposition Authors: Li W, Li A, Yu B, Zhang X
,
Liu X,
White K,
Stevens R,
Baumeister W
,
Sali A
,
Jasnin M
,
Sun L
Sample: INS-1E cell
Deposition Authors: Li W, Li A, Yu B, Zhang X
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In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography.
Li W,
Li A,
Yu B,
Zhang X
,
Liu X,
White KL,
Stevens RC,
Baumeister W
,
Sali A
,
Jasnin M
,
Sun L
(2024) Nat Commun , 15 , 1311 - 1311
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(2024) Nat Commun , 15 , 1311 - 1311
Abstract:
Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a "netlike" to a "blooming" architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion.
Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a "netlike" to a "blooming" architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion.