EMD-41625

Single-particle
3.6 Å
EMD-41625 Deposition: 16/08/2023
Map released: 13/03/2024
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-41625

Type IV pilus from Pseudomonas PAO1 strain

EMD-41625

Single-particle
3.6 Å
EMD-41625 Deposition: 16/08/2023
Map released: 13/03/2024
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO1
Sample: Type IV twitching pilus
Fitted models: 8tum (Avg. Q-score: 0.448)

Deposition Authors: Thongchol J , Zhang J , Zeng L
Removal of Pseudomonas type IV pili by a small RNA virus.
Thongchol J , Yu Z , Harb L , Lin Y, Koch M , Theodore M , Narsaria U, Shaevitz J , Gitai Z , Wu Y, Zhang J , Zeng L
(2024) Science , 384 , eadl0635 - eadl0635
PUBMED: 38574145
DOI: doi:10.1126/science.adl0635
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen Pseudomonas aeruginosa. The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.