EMD-41845

Composite map
Single-particle
2.5 Å
EMD-41845 Deposition: 05/09/2023
Map released: 15/11/2023
Last modified: 09/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-41845

Gaussian mixture model based single particle refinement - ABC transporter (inhibitor-bound ABCG2 from EMPIAR-10374)

EMD-41845

Composite map
Single-particle
2.5 Å
EMD-41845 Deposition: 05/09/2023
Map released: 15/11/2023
Last modified: 09/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens, Mus musculus
Sample: inhibitor-bound ABCG2
Fitted models: 8u2c (Avg. Q-score: 0.534)

Deposition Authors: Chen M
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models.
Chen M , Schmid MF , Chiu W
(2024) Nat Methods , 21 , 37 - 40
PUBMED: 37973972
DOI: doi:10.1038/s41592-023-02082-9
ISSN: 1548-7105
Abstract:
Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.