EMD-45805

Single-particle
3.5 Å
EMD-45805 Deposition: 18/07/2024
Map released: 31/07/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-45805

Infectious bronchitis virus core polymerase complex

EMD-45805

Single-particle
3.5 Å
EMD-45805 Deposition: 18/07/2024
Map released: 31/07/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Infectious bronchitis virus, Severe acute respiratory syndrome coronavirus 2
Sample: Core polymerase complex of the infectious bronchitis virus composed of nsp12, nsp8, nsp7, and an dsRNA substrate
Fitted models: 9cpo (Avg. Q-score: 0.477)

Deposition Authors: Hoferle PJ , Anderson TK , Kirchdoerfer RN
A genus-specific nsp12 region impacts polymerase assembly in Alpha- and Gammacoronaviruses.
PUBMED: 39091740
DOI: doi:10.1101/2024.07.23.604833
ISSN: 2692-8205
Abstract:
Coronavirus relevancy for human health has surged over the past 20 years as they have a propensity for spillover into humans from animal reservoirs resulting in pandemics such as COVID-19. The diversity within the Coronavirinae subfamily and high infection frequency in animal species worldwide creates a looming threat that calls for research across all genera within the Coronavirinae subfamily. We sought to contribute to the limited structural knowledge within the Gammacoronavirus genera and determined the structure of the viral core replication-transcription complex (RTC) from Infectious Bronchitis Virus (IBV) using single-particle cryo-EM. Comparison between our IBV structure with published RTC structures from other Coronavirinae genera reveals structural differences across genera. Using in vitro biochemical assays, we characterized these differences and revealed their differing involvement in core RTC formation across different genera. Our findings highlight the value of cross-genera Coronavirinae studies, as they show genera specific features in coronavirus genome replication. A broader knowledge of coronavirus replication will better prepare us for future coronavirus spillovers.