EMD-4681

Single-particle
3.2 Å
EMD-4681 Deposition: 11/03/2019
Map released: 12/06/2019
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4681

The cryo-EM structure of the head of the genome empited bacteriophage phi29

EMD-4681

Single-particle
3.2 Å
EMD-4681 Deposition: 11/03/2019
Map released: 12/06/2019
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Bacillus phage phi29
Sample: Bacillus phage phi29
Fitted models: 6qz0

Deposition Authors: Xu J , Wang D
Structural assembly of the tailed bacteriophage φ29.
Xu J , Wang D, Gui M , Xiang Y
(2019) Nat Commun , 10 , 2366 - 2366
PUBMED: 31147544
DOI: doi:10.1038/s41467-019-10272-3
ISSN: 2041-1723
Abstract:
The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the ϕ29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-Å long, 24-strand β barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration.