EMD-50200

Single-particle
2.5 Å
EMD-50200 Deposition: 30/04/2024
Map released: 04/09/2024
Last modified: 02/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-50200

EVA71 E096A native particle

EMD-50200

Single-particle
2.5 Å
EMD-50200 Deposition: 30/04/2024
Map released: 04/09/2024
Last modified: 02/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Enterovirus A71
Sample: Enterovirus A71
Fitted models: 9f5s (Avg. Q-score: 0.638)

Deposition Authors: Kingston NJ , Stonehouse NJ , Rowlands DJ , Hogle JM , Filman DJ , Snowden JSS
Mechanism of enterovirus VP0 maturation cleavage based on the structure of a stabilised assembly intermediate.
Kingston NJ , Snowden JS , Grehan K , Hall PK , Hietanen EV , Passchier TC , Polyak SJ , Filman DJ , Hogle JM , Rowlands DJ , Stonehouse NJ
(2024) PLoS Pathog , 20 , e1012511 - e1012511
PUBMED: 39298524
DOI: doi:10.1371/journal.ppat.1012511
ISSN: 1553-7374
Abstract:
Molecular details of genome packaging are little understood for the majority of viruses. In enteroviruses (EVs), cleavage of the structural protein VP0 into VP4 and VP2 is initiated by the incorporation of RNA into the assembling virion and is essential for infectivity. We have applied a combination of bioinformatic, molecular and structural approaches to generate the first high-resolution structure of an intermediate in the assembly pathway, termed a provirion, which contains RNA and intact VP0. We have demonstrated an essential role of VP0 E096 in VP0 cleavage independent of RNA encapsidation and generated a new model of capsid maturation, supported by bioinformatic analysis. This provides a molecular basis for RNA-dependence, where RNA induces conformational changes required for VP0 maturation, but that RNA packaging itself is not sufficient to induce maturation. These data have implications for understanding production of infectious virions and potential relevance for future vaccine and antiviral drug design.