EMD-51235

Single-particle
3.9 Å
EMD-51235 Deposition: 02/08/2024
Map released: 09/10/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-51235

Rho-ATP-Psu complex II

EMD-51235

Single-particle
3.9 Å
EMD-51235 Deposition: 02/08/2024
Map released: 09/10/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Escherichia coli, Enterobacteria phage P4
Sample: Complex of E. coli transcription termination factor Rho with ATP and P4 polarity suppression protein Psu
Fitted models: 9gcs (Avg. Q-score: 0.276)

Deposition Authors: Gjorgjevikj D , Wahl MC , Hilal T , Loll B
The Psu protein of phage satellite P4 inhibits transcription termination factor rho by forced hyper-oligomerization.
Gjorgjevikj D , Kumar N , Wang B , Hilal T , Said N , Loll B , Artsimovitch I , Sen R, Wahl MC
(2025) Nat Commun , 16 , 550 - 550
PUBMED: 39788982
DOI: doi:10.1038/s41467-025-55897-9
ISSN: 2041-1723
Abstract:
Many bacteriophages modulate host transcription to favor expression of their own genomes. Phage satellite P4 polarity suppression protein, Psu, a building block of the viral capsid, inhibits hexameric transcription termination factor, ρ, by presently unknown mechanisms. Our cryogenic electron microscopy structures of ρ-Psu complexes show that Psu dimers clamp two inactive, open ρ rings and promote their expansion to higher-oligomeric states. ATPase, nucleotide binding and nucleic acid binding studies revealed that Psu hinders ρ ring closure and traps nucleotides in their binding pockets on ρ. Structure-guided mutagenesis in combination with growth, pull-down, and termination assays further delineated the functional ρ-Psu interfaces in vivo. Bioinformatic analyses revealed that Psu is associated with a wide variety of phage defense systems across Enterobacteriaceae, suggesting that Psu may regulate expression of anti-phage genes. Our findings show that modulation of the ρ oligomeric state via diverse strategies is a pervasive gene regulatory principle in bacteria.