EMD-5141

Single-particle
23.0 Å
EMD-5141 Deposition: 21/11/2009
Map released: 26/04/2010
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5141

Ab initio reconstruction of the E. coli 70S ribosome complex (70S-fMet-tRNAfMet-Phe-tRNAPhe-EF-Tu-GDP-kirromycin) via the asymmetric random-model method.

EMD-5141

Single-particle
23.0 Å
EMD-5141 Deposition: 21/11/2009
Map released: 26/04/2010
Last modified: 06/03/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Escherichia coli
Sample: E. coli 70S ribosome complex, 70S-fMet-tRNAfMet-Phe-tRNAPhe-EF-Tu-GDP-kirromycin

Deposition Authors: Sanz E, Stewart AB, Belnap DM
The random-model method enables ab initio 3D reconstruction of asymmetric particles and determination of particle symmetry.
Sanz Garcia E , Stewart AB, Belnap DM
(2010) J Struct Biol , 171 , 216 - 222
Abstract:
Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models directly from un-averaged, experimental images of asymmetric or symmetric particles. Therefore, the asymmetric RMM can also be used to determine point-group symmetry. The procedure is facilitated by the use of (a) variable angular step-sizes during iterative origin and orientation searches, (b) high numbers of particle images, and (c) highly defocused images. The method is inhibited by mixed-handedness orientation assignments and by particles with inconspicuous features. For symmetric particles, symmetric RMMs can overcome these deficiencies.