EMD-7468

Single-particle
3.7 Å
EMD-7468 Deposition: 17/02/2018
Map released: 23/05/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7468

cryoEM structure of a respiratory membrane-bound hydrogenase

EMD-7468

Single-particle
3.7 Å
EMD-7468 Deposition: 17/02/2018
Map released: 23/05/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Pyrococcus furiosus COM1, Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1), Pyrococcus furiosus
Sample: Membrane-bound Hydrogenase (MBH) complex
Fitted models: 6cfw (Avg. Q-score: 0.499)

Deposition Authors: Li HL, Yu HJ
Structure of an Ancient Respiratory System.
Yu H , Wu CH, Schut GJ , Haja DK, Zhao G, Peters JW, Adams MWW , Li H
(2018) Cell , 173 , 1636
PUBMED: 29754813
DOI: doi:10.1016/j.cell.2018.03.071
ISSN: 1097-4172
Abstract:
Hydrogen gas-evolving membrane-bound hydrogenase (MBH) and quinone-reducing complex I are homologous respiratory complexes with a common ancestor, but a structural basis for their evolutionary relationship is lacking. Here, we report the cryo-EM structure of a 14-subunit MBH from the hyperthermophile Pyrococcus furiosus. MBH contains a membrane-anchored hydrogenase module that is highly similar structurally to the quinone-binding Q-module of complex I while its membrane-embedded ion-translocation module can be divided into a H+- and a Na+-translocating unit. The H+-translocating unit is rotated 180° in-membrane with respect to its counterpart in complex I, leading to distinctive architectures for the two respiratory systems despite their largely conserved proton-pumping mechanisms. The Na+-translocating unit, absent in complex I, resembles that found in the Mrp H+/Na+ antiporter and enables hydrogen gas evolution by MBH to establish a Na+ gradient for ATP synthesis near 100°C. MBH also provides insights into Mrp structure and evolution of MBH-based respiratory enzymes.