EMD-7884

Single-particle
3.71 Å
EMD-7884 Deposition: 20/05/2018
Map released: 06/11/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7884

BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP1 fragment antigen binding and base-binding RM20A3 fragment antigen binding

EMD-7884

Single-particle
3.71 Å
EMD-7884 Deposition: 20/05/2018
Map released: 06/11/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens, Macaca mulatta, Human immunodeficiency virus 1
Sample: BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP1 fragment antigen binding and base-binding RM20A3 fragment antigen binding

Deposition Authors: Torres JL, Ozorowski G, Steichen JM, Schief WR, Ward AB
A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses.
PUBMED: 31672916
DOI: doi:10.1126/science.aax4380
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Vaccine induction of broadly neutralizing antibodies (bnAbs) to HIV remains a major challenge. Germline-targeting immunogens hold promise for initiating the induction of certain bnAb classes; yet for most bnAbs, a strong dependence on antibody heavy chain complementarity-determining region 3 (HCDR3) is a major barrier. Exploiting ultradeep human antibody sequencing data, we identified a diverse set of potential antibody precursors for a bnAb with dominant HCDR3 contacts. We then developed HIV envelope trimer-based immunogens that primed responses from rare bnAb-precursor B cells in a mouse model and bound a range of potential bnAb-precursor human naïve B cells in ex vivo screens. Our repertoire-guided germline-targeting approach provides a framework for priming the induction of many HIV bnAbs and could be applied to most HCDR3-dominant antibodies from other pathogens.