EMD-8218

Electron Crystallography
2.3 Å
EMD-8218 Deposition: 26/05/2016
Map released: 22/06/2016
Last modified: 28/02/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8218

MicroED structure of xylanase at 2.3 A resolution

EMD-8218

Electron Crystallography
2.3 Å
EMD-8218 Deposition: 26/05/2016
Map released: 22/06/2016
Last modified: 28/02/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Hypocrea jecorina
Sample: Xylanase
Fitted models: 5k7p (Avg. Q-score: 0.724)

Deposition Authors: de la Cruz MJ , Hattne J
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
PUBMED: 28192420
DOI: doi:10.1038/nmeth.4178
ISSN: 1548-7105
Abstract:
Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.