EMD-8305

Single-particle
15.3 Å
EMD-8305 Deposition: 05/08/2016
Map released: 14/09/2016
Last modified: 03/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8305

Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail (Focused Refinement)

EMD-8305

Single-particle
15.3 Å
EMD-8305 Deposition: 05/08/2016
Map released: 14/09/2016
Last modified: 03/02/2021
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail
Fitted models: 5sva (Avg. Q-score: 0.049)

Deposition Authors: Robinson PJ, Bushnell DA, Kornberg RD
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Robinson PJ, Trnka MJ , Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD
(2016) Cell , 166 , 1411 - 1422.e16
PUBMED: 27610567
DOI: doi:10.1016/j.cell.2016.08.050
ISSN: 1097-4172
Abstract:
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.