EMD-8742

Single-particle
3.3 Å
EMD-8742 Deposition: 24/05/2017
Map released: 14/06/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8742

Thermoplasma acidophilum 20S Proteasome using 200keV with image shift

EMD-8742

Single-particle
3.3 Å
EMD-8742 Deposition: 24/05/2017
Map released: 14/06/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Thermoplasma acidophilum
Sample: Thermoplasma acidophilum 20S proteasome
Fitted models: 5vy4
Raw data: EMPIAR-10186

Deposition Authors: Herzik Jr MA, Wu M , Lander GC
Achieving better-than-3- angstrom resolution by single-particle cryo-EM at 200 keV.
Herzik MA, Wu M , Lander GC
(2017) Nat Methods , 14 , 1075 - 1078
PUBMED: 28991891
DOI: doi:10.1038/nmeth.4461
ISSN: 1548-7105
Abstract:
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.