EMD-9741

Single-particle
3.5 Å
EMD-9741 Deposition: 28/11/2018
Map released: 26/02/2020
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9741

Cryo-EM structure of Murine Norovirus S7 VLP

EMD-9741

Single-particle
3.5 Å
EMD-9741 Deposition: 28/11/2018
Map released: 26/02/2020
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Murine norovirus GV, Murine norovirus GV/NIH-2410/2005/USA
Sample: Murine norovirus GV
Fitted models: 6iuk (Avg. Q-score: 0.467)

Deposition Authors: Song C , Miyazaki N , Iwasaki K, Katayama K , Murata K , Yokoyama M , Miyazaki N , Iwasaki K, Murakami K, Katayama K , Murata K
Dynamic rotation of the protruding domain enhances the infectivity of norovirus.
PUBMED: 32614892
DOI: doi:10.1371/journal.ppat.1008619
ISSN: 1553-7374
Abstract:
Norovirus is the major cause of epidemic nonbacterial gastroenteritis worldwide. Lack of structural information on infection and replication mechanisms hampers the development of effective vaccines and remedies. Here, using cryo-electron microscopy, we show that the capsid structure of murine noroviruses changes in response to aqueous conditions. By twisting the flexible hinge connecting two domains, the protruding (P) domain reversibly rises off the shell (S) domain in solutions of higher pH, but rests on the S domain in solutions of lower pH. Metal ions help to stabilize the resting conformation in this process. Furthermore, in the resting conformation, the cellular receptor CD300lf is readily accessible, and thus infection efficiency is significantly enhanced. Two similar P domain conformations were also found simultaneously in the human norovirus GII.3 capsid, although the mechanism of the conformational change is not yet clear. These results provide new insights into the mechanisms of non-enveloped norovirus transmission that invades host cells, replicates, and sometimes escapes the hosts immune system, through dramatic environmental changes in the gastrointestinal tract.