EMD-17757

Single-particle
2.93 Å
EMD-17757
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Deposition: 28/06/2023
Map released: 07/02/2024
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-17757
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8pm4 summary report (pdf.gz) Map and 8pm4 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the Cas12m-crRNA-target DNA complex

EMD-17757

Single-particle
2.93 Å
EMD-17757
3D View Gallery
Deposition: 28/06/2023
Map released: 07/02/2024
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Gordonia otitidis NBRC 100426, synthetic construct
Sample: Cas12m protein in complex with crRNA and cognate DNA, Gordonia otitidis NBRC 100426 system
Fitted models: 8pm4

Deposition Authors: Sasnauskas G, Tamulaitiene G , Karvelis T , Bigelyte G, Siksnys V
Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing.
Bigelyte G, Duchovska B , Zedaveinyte R , Sasnauskas G, Sinkunas T , Dalgediene I , Tamulaitiene G , Silanskas A, Kazlauskas D , Valancauskas L , Madariaga-Marcos J , Seidel R , Siksnys V , Karvelis T
(2024) Nucleic Acids Res , 52 , 3234 - 3248
PUBMED: 38261981
DOI: doi:10.1093/nar/gkae016
ISSN: 1362-4962
ASTM: NARHAD