Structure of yeast RNA Polymerase III Delta C53-C37-C11
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7z1n
Q-score: 0.405
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ Pubmed: 36070694 DOI: doi:10.1016/j.celrep.2022.111316 ]
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (7 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Chapso (631 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii delta c53-c37-c11 (635 kDa, Complex from Saccharomyces cerevisiae W303)
Structure of yeast RNA Polymerase III PTC + NTPs
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7z1o
Q-score: 0.534
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ DOI: doi:10.1016/j.celrep.2022.111316 Pubmed: 36070694 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ptc + ntps (730 kDa, Complex from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Rna (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
Cryo-EM structure of human RNA polymerase I in elongation state
Misiaszek AD, Girbig M, Grötsch H, Baudin F, Murciano B, Lafita A, Müller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ Pubmed: 34887565 DOI: 10.1038/s41594-021-00693-4 ]
Image sets:
Cryo-EM structure of human RNA Polymerase I in elongation state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7ob9
Q-score: 0.519
Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ Pubmed: 34887565 DOI: doi:10.1038/s41594-021-00693-4 ]
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Human)
- Dna non-template strand (13 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Human)
- Rna (9 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Human)
- Rna polymerase i (Complex)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Human)
- Dna template strand (13 kDa, DNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Rna and dna (Complex from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa43 (37 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Human)
Cryo-EM structure of human RNA Polymerase I in complex with RRN3
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7oba
Q-score: 0.435
Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ Pubmed: 34887565 DOI: doi:10.1038/s41594-021-00693-4 ]
- Rna polymerase i in complex with rrn3 (670 kDa, Complex)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Human)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Human)
- Rna polymerase i-specific transcription initiation factor rrn3 (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Human)
- Rna polymerase i-specific transcription initiation factor rrn3 (74 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa43 (37 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Human)
Cryo-EM structure of human RNA Polymerase I Open Complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7obb
Q-score: 0.401
Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ Pubmed: 34887565 DOI: doi:10.1038/s41594-021-00693-4 ]
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Human)
- Rna polymerase i open complex (600 kDa, Complex)
- Dna (Complex from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Human)
- Dna non-template strand (13 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Human)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Human)
- Dna template strand (13 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa43 (37 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Human)
Cryo-EM structures of human RNA Polymerase III
Girbig M, Misiaszek AD, Vorländer MK, Lafita A, Grötsch H, Baudin F, Bateman A, Müller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ DOI: 10.1038/s41594-020-00555-5 Pubmed: 33558764 ]
Image sets:
- Initially picked particles of human RNA Pol III EC
- Polished particles of human RNA Pol III EC (final reconstruction)
- LZW-TIFF compressed multiframe micrographs of human RNA Pol III EC
- Initially picked particles of human RNA Pol III apo
- Polished particles of human RNA Pol III apo (final reconstruction)
- LZW-TIFF compressed multiframe micrographs of human RNA Pol III apo
Cryo-EM structure of human apo RNA Polymerase III
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7a6h
Q-score: 0.425
Girbig M, Misiaszek AD, Vorlander MK, Lafita A, Grotsch H, Baudin F, Bateman A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ Pubmed: 33558764 DOI: doi:10.1038/s41594-020-00555-5 ]
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc2 (127 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Cryo-em structure of human apo rna polymerase iii (776 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc4 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc1 (155 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc7 (25 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc9 (16 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc3 (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc5 (80 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc8 (22 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc6 (35 kDa, Protein from Homo sapiens)
Cryo-EM structure of human RNA Polymerase III elongation complex 1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ae1
Q-score: 0.48
Girbig M, Misiaszek AD, Vorlander MK, Lafita A, Grotsch H, Baudin F, Bateman A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ Pubmed: 33558764 DOI: doi:10.1038/s41594-020-00555-5 ]
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Non-template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc2 (127 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc4 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc1 (155 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc7 (25 kDa, Protein from Homo sapiens)
- Template-dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc9 (16 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc3 (60 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Homo sapiens)
- Rna, dna (Complex from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Human rna polymerase iii elongation complex 1 (776 kDa, Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc5 (80 kDa, Protein from Homo sapiens)
- Rna (6 kDa, RNA from synthetic construct)
- Human rna polymerase (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc8 (22 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc6 (35 kDa, Protein from Homo sapiens)