Polymerase module of CPF in complex with Mpe1 and a pre-cleaved CYC1 RNA
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7zgp
Q-score: 0.624
Rodriguez-Molina JB, O'Reilly FJ, Fagarasan H, Sheekey E, Maslen S, Skehel JM, Rappsilber J, Passmore LA
Mol Cell (2022) 82 pp. 2490- [ Pubmed: 35584695 DOI: doi:10.1016/j.molcel.2022.04.021 ]
- Polyadenylation factor subunit 2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Pre-cleaved cyc1 (13 kDa, RNA from Saccharomyces cerevisiae)
- Mrna 3'-end-processing protein yth1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Mpe1 isoform 1 (49 kDa, Protein from Saccharomyces cerevisiae)
- Protein cft1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Polymerase module-mpe1-rna (Complex from Saccharomyces cerevisiae)
Polymerase module of yeast CPF in complex with the yPIM of Cft2
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7zgq
Q-score: 0.582
Rodriguez-Molina JB, O'Reilly FJ, Fagarasan H, Sheekey E, Maslen S, Skehel JM, Rappsilber J, Passmore LA
Mol Cell (2022) 82 pp. 2490- [ DOI: doi:10.1016/j.molcel.2022.04.021 Pubmed: 35584695 ]
- Mrna 3'-end-processing protein yth1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Protein cft1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Cleavage factor two protein 2 (80 kDa, Protein from Saccharomyces cerevisiae)
- Polyadenylation factor subunit 2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Polymerase module-mpe1-cft2(s)-rna (Complex from Saccharomyces cerevisiae)
Polymerase module of yeast CPF in complex with Mpe1, the yPIM of Cft2 and the pre-cleaved CYC1 RNA
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7zgr
Q-score: 0.61
Rodriguez-Molina JB, O'Reilly FJ, Fagarasan H, Sheekey E, Maslen S, Skehel JM, Rappsilber J, Passmore LA
Mol Cell (2022) 82 pp. 2490- [ Pubmed: 35584695 DOI: doi:10.1016/j.molcel.2022.04.021 ]
- Polyadenylation factor subunit 2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Mrna 3'-end-processing protein yth1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Cleavage factor two protein 2 (80 kDa, Protein from Saccharomyces cerevisiae)
- Mpe1 isoform 1 (49 kDa, Protein from Saccharomyces cerevisiae)
- Protein cft1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Pre-cleaved cyc1 (13 kDa, RNA from Saccharomyces cerevisiae)
- Polymerase module-mpe1-ypim-rna (Complex from Saccharomyces cerevisiae)
Cryo-Em structure of the hexameric RUVBL1-RUVBL2 in complex with ZNHIT2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7p6x
Q-score: 0.313
Serna M, Gonzalez-Corpas A, Cabezudo S, Lopez-Perrote A, Degliesposti G, Zarzuela E, Skehel JM, Munoz J, Llorca O
Nucleic Acids Res (2022) 50 pp. 1128-1146 [ Pubmed: 34951455 DOI: doi:10.1093/nar/gkab1267 ]
- Hexameric ruvbl1-ruvbl2 aaa ring in complex with znhit2 (Complex from Homo sapiens)
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of the human R2TP-TTT complex
Pal M, Munoz-Hernandez H, Bjorklund D, Zhou L, Degliesposti G, Skehel JM, Hesketh EL, Thompson RF, Pearl LH, Llorca O, Prodromou C
Cell Rep (2021) 36 pp. 109317-109317 [ Pubmed: 34233195 DOI: doi:10.1016/j.celrep.2021.109317 ]
Cryo-EM structure of the TELO2-TTI1-TTI2-RUVBL1-RUVBL2 complex
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7ole
Q-score: 0.143
Pal M, Munoz-Hernandez H, Bjorklund D, Zhou L, Degliesposti G, Skehel JM, Hesketh EL, Thompson RF, Pearl LH, Llorca O, Prodromou C
Cell Rep (2021) 36 pp. 109317-109317 [ Pubmed: 34233195 DOI: doi:10.1016/j.celrep.2021.109317 ]
- Telomere length regulation protein tel2 homolog (53 kDa, Protein from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Telo2-tti1-tti2-ruvbl1-ruvbl2 complex (580 kDa, Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Telo2-interacting protein 2,telo2-interacting protein 2,tti2 (95 kDa, Protein from Homo sapiens)
- Telo2-interacting protein 1 homolog,telo2-interacting protein 1 homolog,tti1 (176 kDa, Protein from Homo sapiens)
Unaligned movies for FA core complex and symmetric complex
Shakeel S, Rajendra E, Alcón P, O'Reilly F, Chorev DS, Maslen S, Degliesposti G, Russo CJ, He S, Hill CH, Skehel JM, Scheres SHW, Patel KJ, Rappsilber J, Robinson CV, Passmore LA
Nature (2019) 575 pp. 234-237 [ Pubmed: 31666700 DOI: 10.1038/s41586-019-1703-4 ]
Image sets:
Lehman LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 [ Pubmed: 33357431 DOI: 10.1016/j.celrep.2020.108529 ]
Image sets:
- Particles of the high-resolution nucleosome class
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_3
- Particles of the hexasome class
- Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_2
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_2
- Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_1
- Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_1
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6zhx
Q-score: 0.625
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Dna (145-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Crosslinked complex of alc1 regulatory linker and the nucleosome (206 kDa, Complex)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zhy
Q-score: 0.516
Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S
Cell Rep (2020) 33 pp. 108529-108529 [ Pubmed: 33357431 DOI: doi:10.1016/j.celrep.2020.108529 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- A hexasome (nucleosome lacking one of the two h2a-h2b dimers) with its acidic patch bound by the alc1 linker regulatory region. (170 kDa, Complex)
- Dna (Complex from synthetic construct)
- Histones (Complex from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (Complex from Homo sapiens)
- Dna (110-mer) widom 601 sequence (44 kDa, DNA from synthetic construct)
- Chromodomain-helicase-dna-binding protein 1-like (4 kDa, Protein from Homo sapiens)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)