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was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

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author:"Caspy I" AND author_name:"Caspy I" AND release_date:[2022-01-01T00:00:00Z TO 2022-12-31T23:59:59Z] AND resolution:[2 TO 3}

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EMD-13429

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Unstacked compact Dunaliella PSII

Release date: Aug. 17, 2022
Resolution: 2.43 Å
EM Method: Single-particle
Fitted PDBs: 7pi0
Q-score: 0.623

Caspy I, Fadeeva M, Mazor Y, Nelson N

eLife (2023) 12 [ Pubmed: 36799903 DOI: doi:10.7554/eLife.81150 ]

Sample:

Fe (ii) ion (55 Da, Ligand)
Photosystem ii reaction center protein l (4 kDa, Protein from Dunaliella salina)
(1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
Cp29 (21 kDa, Protein from Dunaliella salina)
Chlorophyll a (893 Da, Ligand)
Chlorophyll b (907 Da, Ligand)
Psbo (25 kDa, Protein from Dunaliella salina)
1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Sodium ion (22 Da, Ligand)
2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
(3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
Beta-carotene (536 Da, Ligand)
Photosystem ii cp43 reaction center protein (49 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein i (3 kDa, Protein from Dunaliella salina)
Pheophytin a (871 Da, Ligand)
Sphingosine (299 Da, Ligand)
Photosystem ii reaction center protein t (3 kDa, Protein from Dunaliella salina)
Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Dunaliella salina)
Cytochrome b559 subunit beta (3 kDa, Protein from Dunaliella salina)
Psbu (3 kDa, Protein from Dunaliella salina)
(2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)
Lhcii m3 (24 kDa, Protein from Dunaliella salina)
Psbw (4 kDa, Protein from Dunaliella salina)
Ca-mn4-o5 cluster (339 Da, Ligand)
(1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
Cp26 (26 kDa, Protein from Dunaliella salina)
Cytochrome b559 subunit alpha (8 kDa, Protein from Dunaliella salina)
Water (18 Da, Ligand)
Photosystem ii reaction center protein j (3 kDa, Protein from Dunaliella salina)
1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
Photosystem ii reaction center protein h (7 kDa, Protein from Dunaliella salina)
Diacyl glycerol (625 Da, Ligand)
Psbx (2 kDa, Protein from Dunaliella salina)
Photosystem ii d2 protein (38 kDa, Protein from Dunaliella salina)
Photosystem ii protein d1 (37 kDa, Protein from Dunaliella salina)
Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
Protoporphyrin ix containing fe (616 Da, Ligand)
Chloride ion (35 Da, Ligand)
Phosphatidylethanolamine (734 Da, Ligand)
Photosystem ii reaction center protein ycf12 (3 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein k (4 kDa, Protein from Dunaliella salina)
(3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
Dunaliella salina photosystem ii complex (Complex from Dunaliella salina)
Bicarbonate ion (61 Da, Ligand)
(3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
Psbp (20 kDa, Protein from Dunaliella salina)
Psbm (3 kDa, Protein from Dunaliella salina)
Lhcii m1 (23 kDa, Protein from Dunaliella salina)
Dodecyl-beta-d-maltoside (510 Da, Ligand)
Photosystem ii cp47 reaction center protein (53 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein z (6 kDa, Protein from Dunaliella salina)

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EMD-13430

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Unstacked stretched Dunaliella PSII

Release date: Aug. 17, 2022
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 7pi5
Q-score: 0.542

Caspy I, Fadeeva M, Mazor Y, Nelson N

eLife (2023) 12 [ Pubmed: 36799903 DOI: doi:10.7554/eLife.81150 ]

Sample:

(1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
Cp29 (21 kDa, Protein from Dunaliella salina)
1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
Beta-carotene (536 Da, Ligand)
Photosystem ii cp43 reaction center protein (49 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein h (7 kDa, Protein from Dunaliella salina)
1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
Photosystem ii d2 protein (38 kDa, Protein from Dunaliella salina)
Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
(3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
Photosystem ii reaction center protein k (4 kDa, Protein from Dunaliella salina)
Hypothetical protein (2 kDa, Protein from Dunaliella salina)
Protoporphyrin ix containing fe (616 Da, Ligand)
Lhcii m1 (23 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein z (6 kDa, Protein from Dunaliella salina)
Lhcii m3 (24 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein i (3 kDa, Protein from Dunaliella salina)
Cp26 (26 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein l (4 kDa, Protein from Dunaliella salina)
Glycerol (92 Da, Ligand)
2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
Psbu (3 kDa, Protein from Dunaliella salina)
Dunaliella salina photosystem ii complex (Complex from Dunaliella salina)
(3r)-beta,beta-caroten-3-ol (552 Da, Ligand)
Sodium ion (22 Da, Ligand)
1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Chlorophyll b (907 Da, Ligand)
(3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)
Photosystem ii reaction center protein ycf12 (3 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein m (3 kDa, Protein from Dunaliella salina)
Psbp (20 kDa, Protein from Dunaliella salina)
Chloride ion (35 Da, Ligand)
Trimethyl-[(2~{r})-1-oxidanyl-1-oxidanylidene-4-[(2~{s})-2-[(1~{s})-1-oxidanyloctadecoxy]-3-[(1~{r})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium (773 Da, Ligand)
Psii 6.1 kda protein (4 kDa, Protein from Dunaliella salina)
Cytochrome b559 subunit alpha (8 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein t (3 kDa, Protein from Dunaliella salina)
Pheophytin a (871 Da, Ligand)
Ca-mn4-o5 cluster (339 Da, Ligand)
Bicarbonate ion (61 Da, Ligand)
Photosystem ii cp47 reaction center protein (53 kDa, Protein from Dunaliella salina)
Psbo (25 kDa, Protein from Dunaliella salina)
Chlorophyll a (893 Da, Ligand)
Fe (ii) ion (55 Da, Ligand)
Diacyl glycerol (625 Da, Ligand)
(1~{s})-3,5,5-trimethyl-4-[(1~{e},3~{e},5~{e},7~{e},9~{e},11~{e},13~{e},15~{e},17~{e})-3,7,12,16-tetramethyl-18-[(4~{s})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol (568 Da, Ligand)
Water (18 Da, Ligand)
Chlorophyll a-b binding protein, chloroplastic (23 kDa, Protein from Dunaliella salina)
Cytochrome b559 subunit beta (3 kDa, Protein from Dunaliella salina)
Photosystem ii reaction center protein j (3 kDa, Protein from Dunaliella salina)
Photosystem ii protein d1 (37 kDa, Protein from Dunaliella salina)
1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
Sphingosine (299 Da, Ligand)
(2s)-3-{[(r)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate (453 Da, Ligand)

[Show more]

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EMDB [2]

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