Structure of VRK1 C-terminal tail bound to nucleosome core particle
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7tan
Q-score: 0.56
Budziszewski GR, Zhao Y, Spangler CJ, Kedziora KM, Williams MR, Azzam DN, Skrajna A, Koyama Y, Cesmat AP, Simmons HC, Arteaga EC, Strauss JD, Kireev D, McGinty RK
Nucleic Acids Res (2022) 50 pp. 4355-4371 [ Pubmed: 35390161 DOI: doi:10.1093/nar/gkac198 ]
- Dna (Complex from synthetic construct)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase vrk1 (45 kDa, Protein from Homo sapiens)
- Widom 601 dna (185-mer) (57 kDa, DNA from synthetic construct)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Structure of vrk1 c-terminal tail bound to nucleosome core particle (Complex)
- Widom 601 dna (185-mer) (56 kDa, DNA from synthetic construct)
- Histone h3.2, histone h4, histone h2a type 1, histone h2b type 1-c/e/f/g/i, serine/threonine-protein kinase vrk1 (Complex from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
Uncrosslinked VRK1-nucleosome complex
Budziszewski GR, Zhao Y, Spangler CJ, Kedziora KM, Williams MR, Azzam DN, Skrajna A, Koyama Y, Cesmat AP, Simmons HC, Arteaga EC, Strauss JD, Kireev D, McGinty RK
Nucleic Acids Res (2022) 50 pp. 4355-4371 [ Pubmed: 35390161 DOI: doi:10.1093/nar/gkac198 ]
- Histone h3.2 (Protein from Homo sapiens)
- Structure of vrk1 c-terminal tail bound to nucleosome core particle (Complex)
- Dna (Complex from synthetic construct)
- Histone h3.2, histone h4, histone h2a type 1, histone h2b type 1-c/e/f/g/i, serine/threonine-protein kinase vrk1 (Complex from Homo sapiens)
- Histone h2b (Protein from Homo sapiens)
- Widom 601 dna (185-mer) (DNA from synthetic construct)
- Vaccinia-related kinase 1 (Protein from Homo sapiens)
- Histone h4 (Protein from Homo sapiens)
- Histone h2a (Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7jo9
Q-score: 0.483
Boyer JA, Spangler CJ, Strauss JD, Cesmat AP, Liu P, McGinty RK, Zhang Q
Science (2020) 370 pp. 450-454 [ Pubmed: 32913000 DOI: doi:10.1126/science.abd0609 ]
- Dna (145-mer) (45 kDa, DNA from synthetic construct)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (145-mer) (45 kDa, DNA from synthetic construct)
- Cyclic gmp-amp synthase (42 kDa, Protein from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- 1:1 cgas-nucleosome complex (250 kDa, Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7joa
Q-score: 0.474
Boyer JA, Spangler CJ, Strauss JD, Cesmat AP, Liu P, McGinty RK, Zhang Q
Science (2020) 370 pp. 450-454 [ Pubmed: 32913000 DOI: doi:10.1126/science.abd0609 ]
- 2:1 cgas-nucleosome complex (290 kDa, Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (145-mer) (45 kDa, DNA from synthetic construct)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (145-mer) (45 kDa, DNA from synthetic construct)
- Cyclic gmp-amp synthase (42 kDa, Protein from Mus musculus)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
Boyer JA, Spangler CJ, Strauss JD, Cesmat AP, Liu P, McGinty RK, Zhang Q
Science (2020) 370 pp. 450-454 [ Pubmed: 32913000 DOI: doi:10.1126/science.abd0609 ]
- 1:1 cgas-nucleosome complex (250 kDa, Complex)