Arp2/3 branch junction complex, ADP state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8uxw
Q-score: 0.585
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ Pubmed: 38448439 DOI: doi:10.1038/s41467-024-46179-x ]
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Actin (Cellular component from Gallus gallus)
- Actin-related protein 3 (47 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp2/3 branch complex (adp state) (Complex)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Schizosaccharomyces pombe)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2 (44 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Schizosaccharomyces pombe)
Arp2/3 branch junction complex, BeFx state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uxx
Q-score: 0.519
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ Pubmed: 38448439 DOI: doi:10.1038/s41467-024-46179-x ]
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Actin (Cellular component from Gallus gallus)
- Actin-related protein 3 (47 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Water (18 Da, Ligand)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Schizosaccharomyces pombe)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2 (44 kDa, Protein from Schizosaccharomyces pombe)
- Beryllium trifluoride ion (66 Da, Ligand)
- Arp2/3 branch complex (adp-befx state) (Complex)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Schizosaccharomyces pombe)
Straight actin filament from Arp2/3 branch junction sample (ADP)
Resolution: 2.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uz0
Q-score: 0.575
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ Pubmed: 38448439 DOI: doi:10.1038/s41467-024-46179-x ]
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Actin (Cellular component from Gallus gallus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx)
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uz1
Q-score: 0.489
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ Pubmed: 38448439 DOI: doi:10.1038/s41467-024-46179-x ]
- Actin (Cellular component from Gallus gallus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Beryllium trifluoride ion (66 Da, Ligand)
- Arp2/3 branch complex (adp-befx state) (Complex)
Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8e9b
Q-score: 0.439
Chou SZ, Chatterjee M, Pollard TD
PNAS (2022) 119 pp. e2206722119-e2206722119 [ DOI: doi:10.1073/pnas.2206722119 Pubmed: 36442092 ]
- S. pombe arp2/3 complex in actin branch junction (Complex from Schizosaccharomyces pombe)
- Actin, alpha skeletal muscle (41 kDa, Protein from chicken)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from fission yeast)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- S. pombe arp2/3 complex in actin branch junction (Complex)
- Chicken skeletal muscle actin in branch junction (Complex from Gallus gallus)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from fission yeast)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from fission yeast)
- Actin-related protein 3 (47 kDa, Protein from fission yeast)
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2 (44 kDa, Protein from fission yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from fission yeast)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Fission yeast)
Cryo-EM structure of pyrene-labeled ADP-actin filaments
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 7k20
Q-score: 0.539
Nat Commun (2020) 11 pp. 5897-5897 [ Pubmed: 33214556 DOI: doi:10.1038/s41467-020-19762-1 ]
- Actin, alpha skeletal muscle (41 kDa, Protein from Chicken)
- Pyrene-labeled adp-actin filaments (Complex from Gallus gallus)
- N-(pyren-1-yl)acetamide (259 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of pyrene-labeled ADP-Pi-actin filaments
Resolution: 3.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7k21
Q-score: 0.549
Nat Commun (2020) 11 pp. 5897-5897 [ Pubmed: 33214556 DOI: doi:10.1038/s41467-020-19762-1 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Chicken)
- Phosphate ion (94 Da, Ligand)
- Pyrene-labeled adp-pi-actin filaments (Complex from Gallus gallus)
- N-(pyren-1-yl)acetamide (259 Da, Ligand)
Cryo-EM structure of AMPPNP-actin filaments
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6djm
Q-score: 0.527
PNAS (2019) 116 pp. 4265-4274 [ Pubmed: 30760599 DOI: doi:10.1073/pnas.1807028115 ]
- Actin, alpha skeletal muscle (41 kDa, Protein from Chicken)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Amppnp-actin (Complex from Gallus gallus)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of ADP-Pi-actin filaments
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6djn
Q-score: 0.541
PNAS (2019) 116 pp. 4265-4274 [ Pubmed: 30760599 DOI: doi:10.1073/pnas.1807028115 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Chicken)
- Adp-pi-actin (Complex from Gallus gallus)
- Phosphate ion (94 Da, Ligand)
Cryo-EM structure of ADP-actin filaments
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 6djo
Q-score: 0.478
PNAS (2019) 116 pp. 4265-4274 [ Pubmed: 30760599 DOI: doi:10.1073/pnas.1807028115 ]
- Magnesium ion (24 Da, Ligand)
- Adp-actin (Complex from Gallus gallus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Gallus gallus)