Human UPF1 RNA helicase with AMPPNP
Langer LM, Kurscheidt K, Basquin J, Bonneau F, Iermak I, Basquin C, Conti E
Nucleic Acids Res (2024) 52 pp. 6036-6048 [ Pubmed: 38709891 DOI: doi:10.1093/nar/gkae323 ]
- Upf1, rent1 (Protein from Homo sapiens)
- Upf1 bound to amppnp (124 kDa, Complex from Homo sapiens)
Human UPF1 RNA helicase with AMPPNP and RNA
Langer LM, Kurscheidt K, Basquin J, Bonneau F, Iermak I, Basquin C, Conti E
Nucleic Acids Res (2024) 52 pp. 6036-6048 [ Pubmed: 38709891 DOI: doi:10.1093/nar/gkae323 ]
- Upf1, rent1 (Protein from Homo sapiens)
- Upf1 bound to amppnp (124 kDa, Complex from Homo sapiens)
Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8r8r
Q-score: 0.578
Todesca S, Sandmeir F, Keidel A, Conti E
RNA (2024) 30 pp. 795-806 [ DOI: doi:10.1261/rna.079915.123 Pubmed: 38538052 ]
- Zinc ion (65 Da, Ligand)
- Mpsf with papoa c-terminus peptide (papoac) (Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (47 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Cdna flj50397, highly similar to poly(a) polymerase alpha (2 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*a)-3') (1 kDa, RNA from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 4 (31 kDa, Protein from Homo sapiens)
yeast cytoplasmic exosome-Ski2 complex degrading a RNA substrate
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8qcf
Q-score: 0.603
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ DOI: doi:10.1016/j.molcel.2023.09.037 Pubmed: 37879335 ]
- Exosome complex component rrp45 (34 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp4 (39 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex exonuclease dis3 (114 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (117 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp43 (44 kDa, Protein from Saccharomyces cerevisiae)
- Yeast cytoplasmic exosome-ski2 complex degrading a rna substrate (Complex from Saccharomyces cerevisiae)
- Exosome complex component rrp40 (26 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component ski6 (27 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component csl4 (31 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp42 (29 kDa, Protein from Saccharomyces cerevisiae)
- Rrp46 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component mtr3 (27 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (5'hairpin 60u) (24 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 7 (39 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski238 complex bound to RNA
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8q9t
Q-score: 0.552
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
- Ski238 polyu rna (Complex from Saccharomyces cerevisiae)
- Rna (5'-r(p*up*up*up*u)-3') (9 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski2387 complex in the closed state bound to RNA
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8qca
Q-score: 0.572
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093- [ DOI: doi:10.1016/j.molcel.2023.09.037 Pubmed: 37879335 ]
- Rna (5'-r(p*up*up*up*u)-3') (9 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 7 (28 kDa, Protein from Saccharomyces cerevisiae)
- Ski2delarch387 polyu rna (Complex from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski2387 complex in an intermediate state bound to RNA
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
CryoEM structure of a S. Cerevisiae Ski2387 complex in the open state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8qcb
Q-score: 0.591
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093- [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
- Ski2delarch387 polyu rna (Complex from Saccharomyces cerevisiae)
- Superkiller protein 7 (28 kDa, Protein from Saccharomyces cerevisiae)
S.cerevisiae THO complex from endogenous nuclear mRNPs
Bonneau F, Basquin J, Steigenberger B, Schafer T, Schafer IB, Conti E
Genes Dev (2023) 37 pp. 505-517 [ DOI: doi:10.1101/gad.350630.123 Pubmed: 37399331 ]
- Tho complex dimer (Complex from Saccharomyces cerevisiae)
- Tho complex subunit hpr1 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit mft1 (Protein from Saccharomyces cerevisiae BY4741)
- Protein tex1 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit 2 (Protein from Saccharomyces cerevisiae BY4741)
- Tho complex subunit thp2 (Protein from Saccharomyces cerevisiae BY4741)
Human NEXT dimer - overall reconstruction of the core complex
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7z4y
Q-score: 0.257
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Mol Cell (2022) 82 pp. 2505- [ DOI: doi:10.1016/j.molcel.2022.04.011 Pubmed: 35688157 ]