Electron Microscopy Data Bank

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Single particle cryo-EM of the P140-P110 heterodimer of Mycoplasma genitalium at 3.3 Angstrom resolution.

Release date: Nov. 1, 2023
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8pbx
Q-score: 0.467

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Mgp-operon protein 3 (Complex from Mycoplasmoides genitalium G37)
Adhesin p1 (159 kDa, Protein from Mycoplasmoides genitalium G37)
Mgp-operon protein 3 (115 kDa, Protein from Mycoplasmoides genitalium G37)
P140-p110 heterodimer (Complex)
Adhesin p1 (Complex from Mycoplasmoides genitalium G37)

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EMD-17588

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Single particle cryo-EM of the P140-P110 heterodimer with an alternative conformation in the P140 stalk of Mycoplasma genitalium at a resolution of 3.7 Angstrom.

Release date: Nov. 1, 2023
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8pby
Q-score: 0.32

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Mgp-operon protein 3 (Complex from Mycoplasmoides genitalium G37)
Adhesin p1 (159 kDa, Protein from Mycoplasmoides genitalium G37)
Mgp-operon protein 3 (115 kDa, Protein from Mycoplasmoides genitalium G37)
P140-p110 heterodimer (Complex)
Adhesin p1 (Complex from Mycoplasmoides genitalium G37)

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EMD-17589

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Single particle cryo-EM of the Nap complex of Mycoplasma genitalium in the expanded conformation at 7.3 Angstrom resolution.

Release date: Nov. 1, 2023
Resolution: 7.3 Å
EM Method: Single-particle

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Nap adhesion complex in the expanded conformation (Complex from Mycoplasmoides genitalium G37)

EMD-17590

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Single particle cryo-EM of the Nap adhesion complex of Mycoplasma genitalium in the canonical conformation at 8.3 Angstrom resolution.

Release date: Nov. 1, 2023
Resolution: 8.3 Å
EM Method: Single-particle

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Nap adhesion complex in canonical conformation (Complex from Mycoplasmoides genitalium G37)

EMD-17591

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Sub-tomogram average of the Nap adhesion complex from the human pathogen Mycoplasma genitalium at 11 Angstrom.

Release date: Nov. 1, 2023
Resolution: 11.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8pbz
Q-score: 0.044

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Mgp-operon protein 3 (114 kDa, Protein from Mycoplasmoides genitalium G37)
Mildly lysed m. genitalium cells (Cell from Mycoplasmoides genitalium G37)
Adhesin p1 (159 kDa, Protein from Mycoplasmoides genitalium G37)

EMD-17592

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Sub-tomogram average of the open conformation of the Nap adhesion complex from the human pathogen Mycoplasma genitalium.

Release date: Nov. 1, 2023
Resolution: 17.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8pc0
Q-score: 0.053

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Mgp-operon protein 3 (114 kDa, Protein from Mycoplasmoides genitalium G37)
Water (18 Da, Ligand)
Potassium ion (39 Da, Ligand)
Phosphate ion (94 Da, Ligand)
Mildly lysed m. genitalium cells (Cell from Mycoplasmoides genitalium G37)
Adhesin p1 (159 kDa, Protein from Mycoplasmoides genitalium G37)

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EMD-17593

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Sub-tomogram average of the closed conformation of the Nap adhesion complex from the human pathogen Mycoplasma genitalium.

Release date: Nov. 1, 2023
Resolution: 18.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8pc1
Q-score: 0.045

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Adhesin p1 (159 kDa, Protein from Mycoplasmoides genitalium G37)
Mgp-operon protein 3 (114 kDa, Protein from Mycoplasmoides genitalium G37)
Mildly lysed m. genitalium cells (Cell from Mycoplasmoides genitalium G37)

EMD-18414

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Single particle cryo-EM of the Nap adhesion complex of Mycoplasma genitalium soaked with 6'-SL at 7.7 Angstrom resolution.

Release date: Nov. 1, 2023
Resolution: 7.7 Å
EM Method: Single-particle

Sprankel L, Scheffer MP, Manger S, Ermel UH, Frangakis AS

PLoS Pathog (2023) 19 pp. e1011761-e1011761 [ Pubmed: 37939157 DOI: doi:10.1371/journal.ppat.1011761 ]

Sample:

Nap adhesion complex soaked with 6-sl (Complex from Mycoplasmoides genitalium G37)

EMD-11678

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Cadherin fit into cryo-ET map

Release date: Dec. 23, 2020
Resolution: 26.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7a7d
Q-score: 0.019

Sikora M, Ermel UH, Seybold A, Kunz M, Calloni G, Reitz J, Vabulas RM, Hummer G, Frangakis AS

PNAS (2020) 117 pp. 27132-27140 [ DOI: doi:10.1073/pnas.2004563117 Pubmed: 33067392 ]

Sample:

Desmosome from mouse liver (Cellular component from Homo sapiens)
Desmocollin-2 (60 kDa, Protein from Human)
Desmoglein-2 (61 kDa, Protein from Human)

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