Grimm C, Jungwirth S, Fischer U
To Be Published
- Mrna-capping enzyme regulatory subunit opg124 (33 kDa, Protein from Vaccinia virus)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (Complex from Vaccinia virus)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Non-template dna oligomer and template dna oligomer (Complex from Vaccinia virus)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
Grimm C, Jungwirth S, Fischer U
To Be Published
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Magnesium ion (24 Da, Ligand)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (Complex from Vaccinia virus)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Template dna oligomer (Complex from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
Grimm C, Jungwirth S, Fischer U
To Be Published
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 large subunit (44 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme regulatory subunit opg124 (33 kDa, Protein from Vaccinia virus)
- Mrna-capping enzyme catalytic subunit (96 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus)
- Intermediate transcription factor 3 small subunit (33 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus)
- Template dna oligomer (21 kDa, DNA from Vaccinia virus)
- Zinc ion (65 Da, Ligand)
- Vaccinia dna-directed rna polymerase open promoter intermediate pre-initiation complex (750 kDa, Complex from Vaccinia virus)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus)
- Non-template dna oligomer (21 kDa, DNA from Vaccinia virus)
Cryo-EM structure of the DNA polymerase holoenzyme E9-A20-D4 of vaccinia virus
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8q3r
Q-score: 0.26
Burmeister WP, Boutin L, Balestra AC, Groger H, Ballandras-Colas A, Hutin S, Kraft C, Grimm C, Bottcher B, Fischer U, Tarbouriech N, Iseni F
PLoS Pathog (2024) 20 pp. e1011652-e1011652 [ DOI: doi:10.1371/journal.ppat.1011652 Pubmed: 38768256 ]
- Dna polymerase (120 kDa, Protein from Vaccinia virus Copenhagen)
- Dna polymerase processivity factor component opg148 (49 kDa, Protein from Vaccinia virus Copenhagen)
- E9-a20-d4 dna polymerase holoenzyme (196 MDa, Complex from Vaccinia virus)
- Uracil-dna glycosylase (27 kDa, Protein from Vaccinia virus Copenhagen)
Cryo EM structure of the vaccinia complete RNA polymerase complex lacking the capping enzyme
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 8c8h
Q-score: 0.333
To Be Published
- Early transcription factor 82 kda subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Core protein e11 (14 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Chr17.trna16-glnttg (23 kDa, RNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna polymerase-associated transcription-specificity factor rap94 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia complete rna polymerase complex lacking the capping enzyme (700 kDa, Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Nucleoside triphosphatase i (72 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Water (18 Da, Ligand)
Symmetric hexamer of vaccinia virus DNA helicase D5 residues 323-785
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8apl
Q-score: 0.248
Hutin S, Ling WL, Tarbouriech N, Schoehn G, Grimm C, Fischer U, Burmeister WP
Viruses (2022) 14 [ DOI: doi:10.3390/v14102206 Pubmed: 36298761 ]
- Primase d5 (53 kDa, Protein from Vaccinia virus Copenhagen)
- D5 c-terminal fragment res. 323 - res. 785 (321 kDa, Complex from Vaccinia virus Copenhagen)
Vaccinia virus DNA helicase D5 residues 323-785 hexamer with bound DNA processed in C1
Resolution: 6.6 Å
EM Method: Single-particle
Fitted PDBs: 8apm
Q-score: 0.152
Hutin S, Ling WL, Tarbouriech N, Schoehn G, Grimm C, Fischer U, Burmeister WP
Viruses (2022) 14 [ DOI: doi:10.3390/v14102206 Pubmed: 36298761 ]
- Dna (5'-d(p*tp*gp*ap*tp*tp*cp*gp*g)-3') (Complex from Synthetic construct)
- Primase d5 (53 kDa, Protein from Vaccinia virus Copenhagen)
- Dna (5'-d(p*cp*cp*gp*ap*ap*tp*cp*a)-3') (9 kDa, DNA from synthetic construct)
- Primase-helicase d5 fragment residues 323-785 (Complex from Vaccinia virus Copenhagen)
- Dna (5'-d(p*cp*cp*gp*ap*ap*tp*cp*a)-3') (Complex from Synthetic construct)
- Dna (5'-d(p*tp*gp*ap*tp*tp*cp*gp*g)-3') (9 kDa, DNA from synthetic construct)
- Primase d5 c-terminal fragment res. 323 - res. 785 (325 kDa, Complex)
Atomic structure of the poxvirus transcription pre-initiation complex in the initially melted state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7amv
Q-score: 0.537
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ DOI: doi:10.1038/s41594-021-00655-w Pubmed: 34556871 ]
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent helicase vetfs (73 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Etf large subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus transcription late pre-initiation complex
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7aof
Q-score: 0.505
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ DOI: doi:10.1038/s41594-021-00655-w Pubmed: 34556871 ]
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Protein h4 (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus late initially transcribing complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7aoh
Q-score: 0.543
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ DOI: doi:10.1038/s41594-021-00655-w Pubmed: 34556871 ]
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna (Complex from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (5'-r(p*ap*ap*ap*ap*up*cp*up*ap*up*a)-3') (3 kDa, RNA from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (Complex from Vaccinia virus GLV-1h68)
- Nucleoside triphosphatase i (72 kDa, Protein from Vaccinia virus GLV-1h68)