Structure of the Cas7-11-Csx29-guide RNA complex
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 7y9x
Q-score: 0.59
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Crrna (12 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11, csx29 (Complex from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Rna (Complex)
Structure of the Cas7-11-Csx29-guide RNA-target RNA (no PFS) complex
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7y9y
Q-score: 0.564
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Crrna, target rna (Complex from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Cas7-11, csx29 (Complex)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Rna (38-mer) (12 kDa, RNA from Desulfonema ishimotonii)
- Rna (27-mer) (8 kDa, RNA from Desulfonema ishimotonii)
Structure of the Cas7-11-Csx29-guide RNA-target RNA (non-matching PFS) complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gs2
Q-score: 0.571
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Adenosine monophosphate (347 Da, Ligand)
- Crrna (30 kDa, RNA from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crrna (Complex)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Target rna with 3'-tag (Complex from Desulfonema ishimotonii)
- Cytidine-5'-monophosphate (323 Da, Ligand)
- Cas7-11, csx29 (Complex)
- Zinc ion (65 Da, Ligand)
- Target rna with non-matching pfs (10 kDa, RNA from Desulfonema ishimotonii)