Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8r8r
Q-score: 0.578
Todesca S, Sandmeir F, Keidel A, Conti E
RNA (2024) 30 pp. 795-806 [ DOI: doi:10.1261/rna.079915.123 Pubmed: 38538052 ]
- Zinc ion (65 Da, Ligand)
- Mpsf with papoa c-terminus peptide (papoac) (Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (47 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Cdna flj50397, highly similar to poly(a) polymerase alpha (2 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*a)-3') (1 kDa, RNA from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 4 (31 kDa, Protein from Homo sapiens)
yeast cytoplasmic exosome-Ski2 complex degrading a RNA substrate
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8qcf
Q-score: 0.603
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ DOI: doi:10.1016/j.molcel.2023.09.037 Pubmed: 37879335 ]
- Exosome complex component rrp45 (34 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp4 (39 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex exonuclease dis3 (114 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (117 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp43 (44 kDa, Protein from Saccharomyces cerevisiae)
- Yeast cytoplasmic exosome-ski2 complex degrading a rna substrate (Complex from Saccharomyces cerevisiae)
- Exosome complex component rrp40 (26 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component ski6 (27 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component csl4 (31 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component rrp42 (29 kDa, Protein from Saccharomyces cerevisiae)
- Rrp46 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Exosome complex component mtr3 (27 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (5'hairpin 60u) (24 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 7 (39 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski238 complex bound to RNA
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8q9t
Q-score: 0.552
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
- Ski238 polyu rna (Complex from Saccharomyces cerevisiae)
- Rna (5'-r(p*up*up*up*u)-3') (9 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski2387 complex in the closed state bound to RNA
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8qca
Q-score: 0.572
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093- [ DOI: doi:10.1016/j.molcel.2023.09.037 Pubmed: 37879335 ]
- Rna (5'-r(p*up*up*up*u)-3') (9 kDa, RNA from Saccharomyces cerevisiae)
- Superkiller protein 7 (28 kDa, Protein from Saccharomyces cerevisiae)
- Ski2delarch387 polyu rna (Complex from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
CryoEM structure of a S. Cerevisiae Ski2387 complex in an intermediate state bound to RNA
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093-4105.e7 [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
CryoEM structure of a S. Cerevisiae Ski2387 complex in the open state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8qcb
Q-score: 0.591
Keidel A, Kogel A, Reichelt P, Kowalinski E, Schafer IB, Conti E
Mol Cell (2023) 83 pp. 4093- [ Pubmed: 37879335 DOI: doi:10.1016/j.molcel.2023.09.037 ]
- Superkiller protein 3 (164 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral protein ski8 (44 kDa, Protein from Saccharomyces cerevisiae)
- Antiviral helicase ski2 (146 kDa, Protein from Saccharomyces cerevisiae)
- Ski2delarch387 polyu rna (Complex from Saccharomyces cerevisiae)
- Superkiller protein 7 (28 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qdy
Q-score: 0.483
Kogel A, Keidel A, Bonneau F, Schafer IB, Conti E
Mol Cell (2022) 82 pp. 756- [ DOI: doi:10.1016/j.molcel.2022.01.009 Pubmed: 35120588 ]
- Helicase ski2w (137 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 61 (33 kDa, Protein from Homo sapiens)
- Tetratricopeptide repeat protein 37 (178 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*up*up*up*up*up*u)-3') (7 kDa, RNA from Homo sapiens)
- H.s. ski complex bound to rna (380 kDa, Complex from Homo sapiens)
80S-bound human SKI complex in the closed state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7qdz
Q-score: 0.396
Kogel A, Keidel A, Bonneau F, Schafer IB, Conti E
Mol Cell (2022) 82 pp. 756- [ Pubmed: 35120588 DOI: doi:10.1016/j.molcel.2022.01.009 ]
- Wd repeat-containing protein 61 (33 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*up*up*up*up*up*u)-3') (1 kDa, RNA from Homo sapiens)
- Helicase ski2w (137 kDa, Protein from Homo sapiens)
- Tetratricopeptide repeat protein 37 (178 kDa, Protein from Homo sapiens)
- H.s. ski complex bound to the ribosome in closed state (380 kDa, Complex from Homo sapiens)
80S-bound human SKI complex in the open state
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 7qe0
Q-score: 0.309
Kogel A, Keidel A, Bonneau F, Schafer IB, Conti E
Mol Cell (2022) 82 pp. 756- [ DOI: doi:10.1016/j.molcel.2022.01.009 Pubmed: 35120588 ]
- Helicase ski2w (137 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*up*up*up*up*up*up*up*up*u)-3') (2 kDa, RNA from Homo sapiens)
- H.s. ski complex bound to the ribosome in open state (380 kDa, Complex from Homo sapiens)
Apo human SKI complex in the closed state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7qdr
Q-score: 0.463
Kogel A, Keidel A, Bonneau F, Schafer IB, Conti E
Mol Cell (2022) 82 pp. 756- [ Pubmed: 35120588 DOI: doi:10.1016/j.molcel.2022.01.009 ]
- H.s. ski complex in the apo closed state (380 kDa, Complex from Homo sapiens)
- Helicase ski2w (137 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 61 (33 kDa, Protein from Homo sapiens)
- Tetratricopeptide repeat protein 37 (178 kDa, Protein from Homo sapiens)