CryoEM reconstruction of tri-specific 298-52-80 Multabody (no symmetry applied)
Burn Aschner C, Muthuraman K, Kucharska I, Cui H, Prieto K, Nair MS, Wang M, Huang Y, Christie-Holmes N, Poon B, Lam J, Sultana A, Kozak R, Mubareka S, Rubinstein JL, Rujas E, Treanor B, Ho DD, Jetha A, Julien JP
Sci Transl Med (2023) 15 pp. eadf4549-eadf4549 [ Pubmed: 37224226 DOI: doi:10.1126/scitranslmed.adf4549 ]
CryoEM reconstruction of the tri-specific 298-52-80 Multabody (octahedral symmetry applied)
Burn Aschner C, Muthuraman K, Kucharska I, Cui H, Prieto K, Nair MS, Wang M, Huang Y, Christie-Holmes N, Poon B, Lam J, Sultana A, Kozak R, Mubareka S, Rubinstein JL, Rujas E, Treanor B, Ho DD, Jetha A, Julien JP
Sci Transl Med (2023) 15 pp. eadf4549-eadf4549 [ Pubmed: 37224226 DOI: doi:10.1126/scitranslmed.adf4549 ]
Structure of HIV-1 capsid declination
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 7urn
Q-score: 0.435
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O
Nat Struct Mol Biol (2023) 30 pp. 383-390 [ DOI: doi:10.1038/s41594-022-00913-5 Pubmed: 36759579 ]
T=1 particle HIV-1 CA G60A/G61P/M66A
Resolution: 2.39 Å
EM Method: Single-particle
Fitted PDBs: 7urt
Q-score: 0.597
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O
Nat Struct Mol Biol (2023) 30 pp. 383-390 [ DOI: doi:10.1038/s41594-022-00913-5 Pubmed: 36759579 ]
T=1 particle HIV-1 CA G60A/G61P
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8eep
Q-score: 0.593
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O
Nat Struct Mol Biol (2023) 30 pp. 383-390 [ Pubmed: 36759579 DOI: doi:10.1038/s41594-022-00913-5 ]
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8eet
Q-score: 0.468
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O
Nat Struct Mol Biol (2023) 30 pp. 383-390 [ DOI: doi:10.1038/s41594-022-00913-5 Pubmed: 36759579 ]
- T=1 particle of hiv-1 ca m66a (Complex from Human immunodeficiency virus 1)
- Gag polyprotein (25 kDa, Protein from Human immunodeficiency virus 1)
- Inositol hexakisphosphate (660 Da, Ligand)
Structure of HIV-1 capsid declination in complex with CPSF6-FG peptide
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8ejl
Q-score: 0.434
Schirra RT, Dos Santos NFB, Zadrozny KK, Kucharska I, Ganser-Pornillos BK, Pornillos O
Nat Struct Mol Biol (2023) 30 pp. 383-390 [ Pubmed: 36759579 DOI: doi:10.1038/s41594-022-00913-5 ]
- Capsid-like particles of in vitro assembled hiv-1 ca protein in complex with cpsf6-fg peptide (Complex from Human immunodeficiency virus 1)
- Hiv-1 capsid protein (25 kDa, Protein from Human immunodeficiency virus 1)
- Cleavage and polyadenylation specificity factor subunit 6 (1 kDa, Protein from Homo sapiens)
Complex of Plasmodium falciparum circumsporozoite protein with 850 Fab
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7v05
Q-score: 0.381
Kucharska I, Binter S, Murugan R, Scally SW, Ludwig J, Prieto K, Thai E, Costa G, Li K, Horn GQ, Flores-Garcia Y, Bosch A, Sicard T, Rubinstein JL, Zavala F, Dennison SM, Tomaras GD, Levashina EA, Kellam P, Wardemann H, Julien JP
PLoS Pathog (2022) 18 pp. e1010999-e1010999 [ Pubmed: 36441829 DOI: doi:10.1371/journal.ppat.1010999 ]
- Plasmodium falciparum circumsporozoite protein (Complex from Plasmodium falciparum)
- 850 fab light chain (23 kDa, Protein from Mus musculus)
- Complex of plasmodium falciparum circumsporozoite protein with 850 fab. (Complex)
- Circumsporozoite protein (39 kDa, Protein from Plasmodium falciparum)
- 850 fab heavy chain (24 kDa, Protein from Mus musculus)
- 850 fab (Complex from Mus musculus)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6x87
Q-score: 0.365
Kucharska I, Thai E, Srivastava A, Rubinstein JL, Pomes R, Julien JP
eLife (2020) 9 [ Pubmed: 33253113 DOI: doi:10.7554/eLife.59018 ]
- 3d11 fab kappa chain (24 kDa, Protein from Mus musculus)
- Complex of plasmodium berghei csp protein and 3d11 fab (Complex)
- 3d11 fab heavy chain, 3d11 fab kappa chain (Complex from Mus musculus)
- 3d11 fab heavy chain (22 kDa, Protein from Mus musculus)
- Circumsporozoite protein (Complex from Plasmodiidae sp.)
- Circumsporozoite protein (36 kDa, Protein from Plasmodium berghei)
Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP
Nat Commun (2021) 12 pp. 3661-3661 [ DOI: doi:10.1038/s41467-021-23825-2 Pubmed: 34135340 ]
- Receptor binding domain of sars-cov-2 spike (Protein from Severe acute respiratory syndrome coronavirus 2)
- Fab fragment of neutralizing antibody 46 (Complex from Homo sapiens)
- Sars-cov-2 receptor binding domain (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab 46 light chain (Protein from Homo sapiens)
- Sars-cov-2 receptor binding domain in complex with the fab fragment of neutralizing antibody 46 (Complex)
- Fab 46 heavy chain (Protein from Homo sapiens)