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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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author:"Mancia F" AND author_name:"Roper DI" AND release_date:[2022-01-01T00:00:00Z TO 2022-12-31T23:59:59Z] AND assembly_molecular_weight:{50000 TO 100000] AND sample_type:"complex" AND author_name:"Stansfeld PJ" AND author_name:"Zinkle AP" AND author_orcid:"0000-0001-6134-1800" AND author_orcid:"0000-0001-8694-7681" AND author_orcid:"0000-0002-9798-4768" AND sample_type:"ligand" AND author_orcid:"0000-0003-0009-621x"

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EMD-26054

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Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state

Release date: April 6, 2022
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497

Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F

Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]

Sample:

Fab light (l) chain (23 kDa, Protein from Homo sapiens)
Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
Ligand bound cmwaal with fab fragment bound (Complex)
Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
Geranyl diphosphate (314 Da, Ligand)

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