Structure of Machupo virus L polymerase in complex with Z protein and 3'-vRNA (dimeric complex)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7el9
Q-score: 0.415
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ DOI: doi:10.1038/s41564-021-00916-w Pubmed: 34127846 ]
- Ring finger protein z (10 kDa, Protein from Machupo mammarenavirus)
- Machupo virus 3'-vrna promoter (Complex from Machupo mammarenavirus)
- Machupo virus 3'-vrna promoter (6 kDa, RNA from Machupo mammarenavirus)
- Manganese (ii) ion (54 Da, Ligand)
- Machupo virus polymerase (Complex)
- Machupo virus polymerase in complex with z martix protein and 3'-vrna in dimeric form. (580 kDa, Complex from Machupo mammarenavirus)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo mammarenavirus)
- Machupo virus z matrix protein (Complex)
Structure of Lassa virus polymerase in complex with 3'-vRNA and Z mutant (F36A)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ela
Q-score: 0.447
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ Pubmed: 34127846 DOI: doi:10.1038/s41564-021-00916-w ]
- Manganese (ii) ion (54 Da, Ligand)
- Lassa virus polymerase in complex with 3'-vrna and z mutant (f36a). (270 kDa, Complex from Lassa mammarenavirus)
- 3-'vrna promoter (Complex from Lassa mammarenavirus)
- Lassa virus z protein (Complex)
- Rna-directed rna polymerase l (253 kDa, Protein from Lassa mammarenavirus)
- 3-'vrna promoter (6 kDa, RNA from Lassa mammarenavirus)
- Ring finger protein z (10 kDa, Protein from Lassa mammarenavirus)
- Zinc ion (65 Da, Ligand)
- Lassa virus polymerase (Complex)
Structure of Machupo virus L polymerase in complex with Z protein (dimeric form)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7elb
Q-score: 0.229
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ Pubmed: 34127846 DOI: doi:10.1038/s41564-021-00916-w ]
- Manganese (ii) ion (54 Da, Ligand)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo mammarenavirus)
- Machupo virus z matrix protein (Complex)
- Ring finger protein z (10 kDa, Protein from Machupo mammarenavirus)
- Machupo virus polymerase in complex with z protein (dimeric form). (580 kDa, Complex from Machupo mammarenavirus)
- Machupo virus polymerase (Complex from Machupo mammarenavirus)
- Zinc ion (65 Da, Ligand)
Structure of monomeric complex of MACV L bound to Z and 3'-vRNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7elc
Q-score: 0.501
Xu X, Peng R, Peng Q, Wang M, Xu Y, Liu S, Tian X, Deng H, Tong Y, Hu X, Zhong J, Wang P, Qi J, Gao GF, Shi Y
Nat Microbiol (2021) 6 pp. 921-931 [ Pubmed: 34127846 DOI: doi:10.1038/s41564-021-00916-w ]
- 3'-vrna promoter (6 kDa, RNA from Machupo mammarenavirus)
- Machupo virus z martix protein (Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo mammarenavirus)
- Zinc ion (65 Da, Ligand)
- Ring finger protein z (10 kDa, Protein from Machupo mammarenavirus)
- 3'-vrna promoter (Complex)
- Machupo virus polymerse (Complex from Machupo mammarenavirus)
- Machupo virus polymerase in complex with z matrix protein and 3'-vrna in monomeric form (270 kDa, Complex from Machupo mammarenavirus)
Structure of apo Lassa virus polymerase
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6klc
Q-score: 0.362
Peng R, Xu X, Jing J, Wang M, Peng Q, Liu S, Wu Y, Bao X, Wang P, Qi J, Gao GF, Shi Y
Nature (2020) 579 pp. 615-619 [ DOI: doi:10.1038/s41586-020-2114-2 Pubmed: 32214249 ]
- Rna-directed rna polymerase l (253 kDa, Protein from Lassa mammarenavirus)
- Lassa virus polymerase (250 kDa, Complex from Lassa mammarenavirus)
- Manganese (ii) ion (54 Da, Ligand)
Structure of apo Machupo virus polymerase
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 6kld
Q-score: 0.46
Peng R, Xu X, Jing J, Wang M, Peng Q, Liu S, Wu Y, Bao X, Wang P, Qi J, Gao GF, Shi Y
Nature (2020) 579 pp. 615-619 [ DOI: doi:10.1038/s41586-020-2114-2 Pubmed: 32214249 ]
- Machupo virus polymerase (250 kDa, Complex from Machupo mammarenavirus)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo virus)
- Zinc ion (65 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
Monomeric structure of Machupo virus polymerase bound to vRNA promoter
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6kle
Q-score: 0.27
Peng R, Xu X, Jing J, Wang M, Peng Q, Liu S, Wu Y, Bao X, Wang P, Qi J, Gao GF, Shi Y
Nature (2020) 579 pp. 615-619 [ DOI: doi:10.1038/s41586-020-2114-2 Pubmed: 32214249 ]
- Machupo virus polymerase bound to vrna promoter (270 kDa, Complex from Machupo mammarenavirus)
- Rna (5'-r(*gp*cp*cp*up*ap*gp*gp*ap*up*cp*cp*ap*cp*up*gp*up*gp*cp*g)-3') (6 kDa, RNA from synthetic construct)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo virus)
- Zinc ion (65 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
Dimeric structure of Machupo virus polymerase bound to vRNA promoter
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6klh
Q-score: 0.321
Peng R, Xu X, Jing J, Wang M, Peng Q, Liu S, Wu Y, Bao X, Wang P, Qi J, Gao GF, Shi Y
Nature (2020) 579 pp. 615-619 [ Pubmed: 32214249 DOI: doi:10.1038/s41586-020-2114-2 ]
- Manganese (ii) ion (54 Da, Ligand)
- Rna-directed rna polymerase l (250 kDa, Protein from Machupo virus)
- Zinc ion (65 Da, Ligand)
- Machupo virus polymerase bound to vrna promoter (540 kDa, Complex from Machupo mammarenavirus)
- Rna (5'-r(*gp*cp*cp*up*ap*gp*gp*ap*up*cp*cp*ap*cp*up*gp*up*gp*cp*g)-3') (6 kDa, RNA from synthetic construct)
structure of influenza D virus apo polymerase
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 6kv5
Q-score: 0.213
Peng Q, Liu Y, Peng R, Wang M, Yang W, Song H, Chen Y, Liu S, Han M, Zhang X, Wang P, Yan J, Zhang B, Qi J, Deng T, Gao GF, Shi Y
Nat Microbiol (2019) [ DOI: doi:10.1038/s41564-019-0487-5 Pubmed: 31209309 ]
- Polymerase 3 (83 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))
- Influenza d virus polymerase (270 kDa, Complex from Thogotovirus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))
- Polymerase pb2 (88 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))
Structure of influenza D virus polymerase bound to vRNA promoter in mode A conformation (class A1)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6kuk
Q-score: 0.403
Peng Q, Liu Y, Peng R, Wang M, Yang W, Song H, Chen Y, Liu S, Han M, Zhang X, Wang P, Yan J, Zhang B, Qi J, Deng T, Gao GF, Shi Y
Nat Microbiol (2019) [ DOI: doi:10.1038/s41564-019-0487-5 Pubmed: 31209309 ]
- Influenza d virus polymerase bound to vrna promoter (290 kDa, Complex from Influenza D virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))
- Polymerase 3 (83 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))
- Rna (5'-r(p*cp*up*cp*cp*up*gp*cp*up*up*ap*up*gp*cp*u)-3') (4 kDa, RNA from synthetic construct)
- Rna (5'-r(p*ap*gp*cp*ap*gp*up*ap*gp*cp*ap*ap*gp*gp*ap*g)-3') (4 kDa, RNA from synthetic construct)
- Polymerase pb2 (88 kDa, Protein from Influenza D virus (D/swine/Oklahoma/1334/2011))