Electron Microscopy Data Bank

EMPIAR-10718

Release date: June 25, 2021
Dataset size: 14.3 TB
Experiment type: EMDB

Lim C, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: 10.1126/science.aaz9649 Pubmed: 32499435 ]

Image sets:

EMD-21561

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The structure of human CST reveals a decameric assembly bound to telomeric DNA

Release date: June 3, 2020
Resolution: 3.0 Å
EM Method: Single-particle

Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]

Sample:

Homo sapiens cst complex subunit ctc1 (Protein from Homo sapiens)
3xtel (DNA from Homo sapiens)
Decameric assembly of human cst complex with telomeric single-stranded dna (2 MDa, Complex from Homo sapiens)
Homo sapiens cst complex subunit ten1 (Protein from Homo sapiens)
Homo sapiens cst complex subunit stn1 (Protein from Homo sapiens)

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EMD-21563

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DNA-free CST monomer

Release date: June 3, 2020
Resolution: 6.3 Å
EM Method: Single-particle

Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]

Sample:

Heterotrimeric complex of human cst complex without dna (200 kDa, Complex from Homo sapiens)
Homo sapien stn1 (Protein from Homo sapiens)
Homo sapien ctc1 (Protein from Homo sapiens)
Homo sapien ten1 (Protein from Homo sapiens)

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EMD-21565

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Arm conformation from CST-3xTEL oligomer-mixture

Release date: June 3, 2020
Resolution: 9.2 Å
EM Method: Single-particle

Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]

Sample:

Arm conformation of cst monomer from cst-3xtel oligomer-mixture (200 kDa, Complex from Homo sapiens)

EMD-21566

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Head conformation from CST-3xTEL oligomer-mixture

Release date: June 3, 2020
Resolution: 8.9 Å
EM Method: Single-particle

Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]

Sample:

Head conformation of cst monomer from cst-3xtel oligomer-mixture (200 kDa, Complex from Homo sapiens)

EMD-21567

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Cryo-EM structure of CST bound to telomeric single-stranded DNA

Release date: June 3, 2020
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6w6w
Q-score: 0.536

Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]

Sample:

Dna (5'-d(p*tp*ap*gp*g)-3') (1 kDa, DNA from Homo sapiens)
Cst complex subunit stn1 (43 kDa, Protein from Homo sapiens)
Human cst complex (200 kDa, Complex from Homo sapiens)
Cst complex subunit ten1 (17 kDa, Protein from Homo sapiens)
Zinc ion (65 Da, Ligand)
Cst complex subunit ctc1 (136 kDa, Protein from Homo sapiens)

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