Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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author:"Xu W" AND author_orcid:"0000-0001-9866-2384" AND film_detector_model:"GATAN K3 (6K X 4K)" AND author_name:"Wang M" AND resolution:[3 TO 4}

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EMD-30219

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Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with di-arabinose.

Release date: April 29, 2020
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7bvg
Q-score: 0.537

Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z

Science (2020) 368 pp. 1211-1219 [ Pubmed: 32327601 DOI: doi:10.1126/science.aba9102 ]

Sample:

Mycobacterium smegmatis arabinosyltransferase emba-embb-acpm2 in complex with di-arabinose (Complex)
[(2z,6e,10e,14z,18e,22z,26z)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl] [(2s,3s,4s,5r)-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl] hydrogen phosphate (911 Da, Ligand)
Integral membrane indolylacetylinositol arabinosyltransferase embb (119 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Cardiolipin (1 kDa, Ligand)
Emba and embb (Complex from Mycolicibacterium smegmatis MC2 155)
Acpm (Complex from Mycolicibacterium smegmatis MC2 155)
Integral membrane indolylacetylinositol arabinosyltransferase emba (116 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Meromycolate extension acyl carrier protein (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Calcium ion (40 Da, Ligand)
Phosphate ion (94 Da, Ligand)
4'-phosphopantetheine (358 Da, Ligand)

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