Structure of HIV-1 reverse transcriptase initiation complex core
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7kjv
Q-score: 0.564
Ha B, Larsen KP, Zhang J, Fu Z, Montabana E, Jackson LN, Chen DH, Puglisi EV
Nat Commun (2021) 12 pp. 2500-2500 [ Pubmed: 33947853 DOI: doi:10.1038/s41467-021-22628-9 ]
- Hiv-1 reverse transcriptase initiation complex core (138 kDa, Complex from Human immunodeficiency virus 1)
- Trna lysine 3 fragment with gnra tetraloop (12 kDa, Macromolecule from Homo sapiens)
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- Octyl beta-d-glucopyranoside (292 Da, Ligand)
- Reverse transcriptase/ribonuclease h (64 kDa, Protein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10))
- Hiv-1 viral rna fragment (8 kDa, RNA from Human immunodeficiency virus 1)
Structure of HIV-1 reverse transcriptase initiation complex core with efavirenz
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7kjw
Q-score: 0.55
Ha B, Larsen KP, Zhang J, Fu Z, Montabana E, Jackson LN, Chen DH, Puglisi EV
Nat Commun (2021) 12 pp. 2500-2500 [ DOI: doi:10.1038/s41467-021-22628-9 Pubmed: 33947853 ]
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Trna lysine 3 fragment with gnra tetraloop (12 kDa, Macromolecule from Homo sapiens)
- (-)-6-chloro-4-cyclopropylethynyl-4-trifluoromethyl-1,4-dihydro-2h-3,1-benzoxazin-2-one (315 Da, Ligand)
- Hiv-1 reverse transcriptase initiation complex core with efavirenz (138 kDa, Complex from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- Reverse transcriptase/ribonuclease h (64 kDa, Protein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10))
- Hiv-1 viral rna fragment (8 kDa, RNA from Human immunodeficiency virus 1)
Structure of HIV-1 reverse transcriptase initiation complex core with nevirapine
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7kjx
Q-score: 0.515
Ha B, Larsen KP, Zhang J, Fu Z, Montabana E, Jackson LN, Chen DH, Puglisi EV
Nat Commun (2021) 12 pp. 2500-2500 [ Pubmed: 33947853 DOI: doi:10.1038/s41467-021-22628-9 ]
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Magnesium ion (24 Da, Ligand)
- Reverse transcriptase/ribonuclease h (64 kDa, Protein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10))
- Hiv-1 reverse transcriptase initiation complex core with nevirapine (138 kDa, Complex from Human immunodeficiency virus 1)
- Trna lysine 3 fragment with gnra tetraloop (7 kDa, Macromolecule from Homo sapiens)
- 11-cyclopropyl-5,11-dihydro-4-methyl-6h-dipyrido[3,2-b:2',3'-e][1,4]diazepin-6-one (266 Da, Ligand)
- Hiv-1 viral rna fragment (8 kDa, RNA from Human immunodeficiency virus 1)
+1 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6waz
Q-score: 0.387
Larsen KP, Choi J, Jackson LN, Kappel K, Zhang J, Ha B, Chen DH, Puglisi EV
J Mol Biol (2020) 432 pp. 4499-4522 [ DOI: doi:10.1016/j.jmb.2020.06.003 Pubmed: 32512005 ]
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Trna lysine 3 (Complex from Homo sapiens)
- Reverse transcriptase/ribonuclease h (64 kDa, Protein from Human immunodeficiency virus 1)
- +1 extended hiv-1 reverse transcriptase initiation complex core (25 kDa, Complex from Human immunodeficiency virus 1)
- Trna lysine 3 (24 kDa, Macromolecule from Homo sapiens)
- Hiv-1 reverse transcriptase (Complex from Human immunodeficiency virus 1)
- Hiv-1 viral rna genome fragment (32 kDa, RNA from Human immunodeficiency virus 1)
- Hiv-1 rna genome fragment (Complex from Human immunodeficiency virus 1)
+3 extended HIV-1 reverse transcriptase initiation complex core (intermediate state)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6wb1
Q-score: 0.304
Larsen KP, Choi J, Jackson LN, Kappel K, Zhang J, Ha B, Chen DH, Puglisi EV
J Mol Biol (2020) 432 pp. 4499-4522 [ DOI: doi:10.1016/j.jmb.2020.06.003 Pubmed: 32512005 ]
- Hiv-1 rna genome fragment (Complex from Human immunodeficiency virus 1)
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Reverse transcriptase/ribonuclease h (64 kDa, Protein from Human immunodeficiency virus type 1 group M subtype B (isolate BH10))
- Hiv-1 reverse transcriptase (Complex from Human immunodeficiency virus 1)
- +3 extended hiv-1 reverse transcriptase initiation complex core (intermediate state) (25 kDa, Complex from Human immunodeficiency virus 1)
- Trna lysine 3 (25 kDa, Macromolecule from Homo sapiens)
- Trna lysine 3 (Complex from Homo sapiens)
- Hiv-1 viral rna genome fragment (32 kDa, RNA from Human immunodeficiency virus 1)
Architecture of HIV-1 reverse transcriptase initiation complex core
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6b19
Q-score: 0.301
Larsen KP, Mathiharan YK, Kappel K, Coey AT, Chen DH, Barrero D, Madigan L, Puglisi JD, Skiniotis G, Puglisi EV
Nature (2018) 557 pp. 118-122 [ Pubmed: 29695867 DOI: doi:10.1038/s41586-018-0055-9 ]
- Hiv-1 reverse transcription initiation complex core (175 kDa, Complex)
- Reverse transcriptase p66 subunit (65 kDa, Protein from Human immunodeficiency virus 1)
- Hiv-1 rna genome fragment (33 kDa, Complex from Human immunodeficiency virus 1)
- Hiv-1 reverse transcriptase (117 kDa, Complex from Human immunodeficiency virus 1)
- Reverse transcriptase p51 subunit (51 kDa, Protein from Human immunodeficiency virus 1)
- Trna lysine3 (24 kDa, Macromolecule from Homo sapiens)
- Rna genome fragment (32 kDa, RNA from Human immunodeficiency virus 1)
- Trna lysine3 primer (25 kDa, Complex from Homo sapiens)