Cryo-EM structure of yeast mitochondrial RNA polymerase transcription initiation complex
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 6ymw
Q-score: 0.41
De Wijngaert B, Sultana S, Singh A, Dharia C, Vanbuel H, Shen J, Vasilchuk D, Martinez SE, Kandiah E, Patel SS, Das K
Mol Cell (2021) 81 pp. 268- [ DOI: doi:10.1016/j.molcel.2020.11.016 Pubmed: 33278362 ]
- Chains: n (10 kDa, DNA from Saccharomyces cerevisiae S288C)
- Chains: t (10 kDa, DNA from Saccharomyces cerevisiae S288C)
- Mitochondria dna-dependent rna polymerase pre-initiation complex (202 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from Saccharomyces cerevisiae)
- Mitochondrial transcription factor 1 (41 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mitochondria dna-dependent rna polymerase pre-initiation complex (Complex from Saccharomyces cerevisiae)
- Rna (pppgpg) (Complex from synthetic construct)
- [[(2~{r},3~{s},4~{r},5~{r})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate (500 Da, Ligand)
- Dna-directed rna polymerase, mitochondrial (143 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna (pppgpg) (805 Da, RNA from synthetic construct)
Cryo-EM structure of yeast mitochondrial RNA polymerase transcription pre-initiation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6ymv
Q-score: 0.536
De Wijngaert B, Sultana S, Singh A, Dharia C, Vanbuel H, Shen J, Vasilchuk D, Martinez SE, Kandiah E, Patel SS, Das K
Mol Cell (2021) 81 pp. 268- [ DOI: doi:10.1016/j.molcel.2020.11.016 Pubmed: 33278362 ]
- Dna (33-mer) template (10 kDa, DNA from Saccharomyces cerevisiae)
- Dna (Complex from Saccharomyces cerevisiae)
- Dna-directed rna polymerase, mitochondrial (143 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mitochondrial transcription factor 1 (41 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mitochondria dna-dependent rna polymerase pre-initiation complex (202 kDa, Complex)
- Mitochondria dna-dependent rna polymerase pre-initiation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (33-mer) non-template (10 kDa, DNA from Saccharomyces cerevisiae)
Electron cryo-microscopy of a transcribing Pol IIp-Capping Enzyme complex
Martinez-Rucobo FW, Kohler R, van de Waterbeemd M, Heck AJR, Hemann M, Herzog F, Stark H, Cramer P
Mol Cell (2015) 58 pp. 1079-1089 [ Pubmed: 25959396 DOI: doi:10.1016/j.molcel.2015.04.004 ]
- Mrna-capping enzyme (180 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii (520 kDa, Protein from Saccharomyces cerevisiae)
- Dna-rna scaffold (20 kDa, Macromolecule from Saccharomyces cerevisiae)
- Transcribing pol iip-capping enzyme complex from yeast (720 kDa, Sample)
Electron cryo-microscopy of a transcribing Pol IIp complex
Martinez-Rucobo FW, Kohler R, van de Waterbeemd M, Heck AJR, Hemann M, Herzog F, Stark H, Cramer P
Mol Cell (2015) 58 pp. 1079-1089 [ Pubmed: 25959396 DOI: doi:10.1016/j.molcel.2015.04.004 ]
- Transcribing pol iip complex from yeast (540 kDa, Sample)
- Dna-directed rna polymerase ii (520 kDa, Protein from Saccharomyces cerevisiae)
- Dna-rna scaffold (20 kDa, Macromolecule from Saccharomyces cerevisiae)