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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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dna/ polymerase AND electron_source:"FIELD EMISSION GUN" AND microscope_name:"TFS KRIOS" AND author_orcid:"0000-0002-7268-9631" AND release_date:[2020-01-01T00:00:00Z TO 2020-12-31T23:59:59Z]

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EMD-11692

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Nsp7-Nsp8-Nsp12 SARS-CoV2 RNA-dependent RNA polymerase in complex with template:primer dsRNA and favipiravir-RTP

Release date: Sept. 23, 2020
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7aap
Q-score: 0.571

Naydenova K, Muir KW, Wu LF, Zhang Z, Coscia F, Peet MJ, Castro-Hartmann P, Qian P, Sader K, Dent K, Kimanius D, Sutherland JD, Lowe J, Barford D, Russo CJ

PNAS (2021) 118 [ Pubmed: 33526596 DOI: doi:10.1073/pnas.2021946118 ]

Sample:

Nsp7-nsp8-nsp12 sars-cov2 rna-directed rna polymerase (Complex from Severe acute respiratory syndrome coronavirus 2)
Rna (Complex from Severe acute respiratory syndrome coronavirus 2)
Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Magnesium ion (24 Da, Ligand)
[[(2~{r},3~{s},4~{r},5~{r})-5-(3-aminocarbonyl-5-fluoranyl-2-oxidanylidene-pyrazin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate (529 Da, Ligand)
Nsp7-nsp8-nsp12 sars-cov2 rna-directed rna polymerase in complex with template:primer dsrna and favipiravir-rtp (Complex)
Zinc ion (65 Da, Ligand)
Rna (5'-r(p*up*up*cp*ap*up*ap*ap*cp*up*up*ap*a)-3') (9 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Rna (5'-r(p*up*up*ap*ap*gp*up*up*ap*u)-3') (7 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Non-structural protein 12 (110 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Pyrophosphate 2- (175 Da, Ligand)

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