Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7uwh
Q-score: 0.458
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ Pubmed: 37196657 DOI: doi:10.1016/j.cell.2023.04.029 ]
- Dna (59-mer) (18 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna/rna (59-mer) (18 kDa, Macromolecule from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Elongation complex(ec)-rnaseh2 complex bound to ribonucleotide substrate (Complex from Escherichia coli)
Abortive infection DNA polymerase AbiK from Lactococcus lactis
Resolution: 2.27 Å
EM Method: Single-particle
Fitted PDBs: 7r06
Q-score: 0.676
Figiel M, Gapinska M, Czarnocki-Cieciura M, Zajko W, Sroka M, Skowronek K, Nowotny M
Nucleic Acids Res (2022) 50 pp. 10026-10040 [ DOI: doi:10.1093/nar/gkac772 Pubmed: 36107766 ]
- Homohexamer of abik with single-stranded dna covalently attached to each of the subunits (420 kDa, Complex)
- Dna (5'-d(*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*c)-3') (3 kDa, DNA from Escherichia coli)
- Abik (71 kDa, Protein from Lactococcus lactis)
- Abortive infection protein abik (Complex from Lactococcus lactis)
- Dna (5'-d(*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*cp*c)-3') (Complex from Escherichia coli)
Cryo-EM structure of E.coli retron-Ec86 (RT-msDNA-RNA) at 3.2 angstrom
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7v9u
Q-score: 0.471
Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S, Zou T
Nat Microbiol (2022) 7 pp. 1480-1489 [ Pubmed: 35982312 DOI: doi:10.1038/s41564-022-01197-7 ]
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (36 kDa, Protein from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna (5'-r(p*cp*gp*up*ap*ap*gp*gp*g)-3') (4 kDa, RNA from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.8 angstrom
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7v9x
Q-score: 0.549
To Be Published
- Retron st85 family effector protein (35 kDa, Protein from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna (14-mer) (4 kDa, RNA from Escherichia coli)
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (37 kDa, Protein from Escherichia coli)
Resolution: 4.35 Å
EM Method: Single-particle
Fitted PDBs: 6pb4
Q-score: 0.224
Shi W, Jiang Y, Deng Y, Dong Z, Liu B
PLoS Biol (2020) 18 pp. e3000706- [ DOI: doi:10.1371/journal.pbio.3000706 ]
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli)
- Dna/rna (Complex)
- Dna-directed rna polymerase subunit alpha, dna-directed rna polymerase subunit beta, dna-directed rna polymerase subunit beta', dna-directed rna polymerase subunit omega, rna polymerase sigma factor rpod, camp-activated global transcriptional regulator crp (Complex from Escherichia coli)
- Synthetic template strand dna (78-mer) (24 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Synthetic nontemplate strand dna (78-mer) (23 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli)
- Nascent rna (1 kDa, RNA from Escherichia coli)
- The e. coli class-ii cap-dependent transcription activation complex with de novo rna transcript at the state 2 (550 kDa, Complex from Escherichia coli)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7w
Q-score: 0.324
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Dna (25-mer) (7 kDa, DNA from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (77-mer) (23 kDa, DNA from Enterobacteria phage T7)
- Trxa (11 kDa, Protein from Escherichia coli)
- D5ae7a mutant gp5 dna polymerase complexed with trx, fork dna, and incoming dttp (from gp4-gp5 leading-strand complexes) (Complex from Enterobacteria phage T7)