Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8ef9
Q-score: 0.605
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2023) 51 pp. 463-474 [ Pubmed: 36583344 DOI: doi:10.1093/nar/gkac1201 ]
- Dna (5'-d(*ap*gp*cp*ap*tp*cp*cp*gp*tp*ap*gp*(2da))-3') (5 kDa, DNA from Lates calcarifer)
- Ternary complex of lates calcarifer dna polymerase theta with duplex dna and incoming nucleotide (Complex from Lates calcarifer)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase theta (95 kDa, Protein from Lates calcarifer)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(*ap*gp*cp*tp*cp*tp*ap*cp*gp*gp*ap*tp*gp*c)-3') (8 kDa, DNA from Lates calcarifer)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8efc
Q-score: 0.492
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2023) 51 pp. 463-474 [ Pubmed: 36583344 DOI: doi:10.1093/nar/gkac1201 ]
- Ternary complex of lates calcarifer dna polymerase theta with duplex dna and incoming nucleotide (Complex from Lates calcarifer)
- Dna (5'-d(*ap*cp*tp*gp*tp*gp*ap*gp*gp*cp*ap*tp*cp*cp*gp*tp*ap*gp*(2da))-3') (6 kDa, DNA from Lates calcarifer)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase theta (95 kDa, Protein from Lates calcarifer)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(*ap*gp*cp*tp*cp*tp*ap*cp*gp*gp*ap*tp*gp*cp*cp*tp*cp*ap*cp*ap*g)-3') (8 kDa, DNA from Lates calcarifer)
SARS-CoV-2 replication-transcription complex bound to GTP, in a pre-catalytic state
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7uob
Q-score: 0.548
Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Gotte M, Chait BT, Campbell EA, Darst SA
Nature (2023) 614 pp. 781-787 [ Pubmed: 36725929 DOI: doi:10.1038/s41586-022-05664-3 ]
- 3'-deoxyuridine-5'-monophosphate (308 Da, Ligand)
- Product rna (35-mer) (11 kDa, RNA from synthetic construct)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Sars-cov-2 replication-transcription complex + gtp (Complex from Severe acute respiratory syndrome coronavirus 2)
- Template rna (55-mer) (17 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 7 (10 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-CoV-2 replication-transcription complex bound to CTP, in a pre-catalytic state
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 7uoe
Q-score: 0.523
Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Gotte M, Chait BT, Campbell EA, Darst SA
Nature (2023) 614 pp. 781-787 [ DOI: doi:10.1038/s41586-022-05664-3 Pubmed: 36725929 ]
- Product rna (35-mer) (11 kDa, RNA from synthetic construct)
- Non-structural protein 7 (10 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Cytidine-5'-triphosphate (483 Da, Ligand)
- Template rna (55-mer) (17 kDa, RNA from synthetic construct)
- Sars-cov-2 replication-transcription complex + ctp (Complex from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- 3'-deoxyuridine-5'-monophosphate (308 Da, Ligand)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
CryoEM Structure of an Group II Intron Retroelement
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7uin
Q-score: 0.467
Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM
Science (2022) 378 pp. 627-634 [ Pubmed: 36356138 DOI: doi:10.1126/science.abq2844 ]
- Magnesium ion (24 Da, Ligand)
- Ternary complex of rna, protein and dna (260 kDa, Complex from [Eubacterium] rectale)
- Water (18 Da, Ligand)
- Ammonium ion (18 Da, Ligand)
- Group ii intron reverse transcriptase/maturase (49 kDa, Protein from [Eubacterium] rectale)
- Dna (37-mer) (11 kDa, DNA from [Eubacterium] rectale)
- E.r iic intron (206 kDa, RNA from [Eubacterium] rectale)
Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.8 angstrom
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7v9x
Q-score: 0.549
To Be Published
- Retron st85 family effector protein (35 kDa, Protein from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna (14-mer) (4 kDa, RNA from Escherichia coli)
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (37 kDa, Protein from Escherichia coli)
A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7ed5
Q-score: 0.522
Shannon A, Fattorini V, Sama B, Selisko B, Feracci M, Falcou C, Gauffre P, El Kazzi P, Delpal A, Decroly E, Alvarez K, Eydoux C, Guillemot JC, Moussa A, Good SS, La Colla P, Lin K, Sommadossi JP, Zhu Y, Yan X, Shi H, Ferron F, Canard B
Nat Commun (2022) 13 pp. 621-621 [ DOI: doi:10.1038/s41467-022-28113-1 Pubmed: 35110538 ]
- Nsp12-nsp7-nsp8 (Complex from Severe acute respiratory syndrome coronavirus 2)
- [[(2r,3r,4r,5r)-5-(2-azanyl-6-oxidanylidene-1h-purin-9-yl)-4-fluoranyl-4-methyl-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate (539 Da, Ligand)
- Nsp12-nsp7-nsp8-rna-at9010 (160 kDa, Complex)
- Rna-directed rna polymerase (109 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (12 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (Complex)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*cp*cp*cp*cp*ap*up*ap*ap*cp*up*up*ap*ap*up*cp*up*cp*ap*cp*ap*up*ap*gp*c)-3') (9 kDa, RNA from synthetic construct)
- Non-structural protein 8 (24 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*gp*cp*up*ap*up*gp*up*gp*ap*gp*ap*up*up*ap*ap*gp*up*up*ap*u*(at9))-3') (6 kDa, RNA from synthetic construct)
Structure of COVID-19 RNA-dependent RNA polymerase bound to penciclovir.
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7doi
Q-score: 0.599
Yin W, Luan X, Li Z, Xie Y, Zhou Z, Liu J, Gao M, Wang X, Zhou F, Wang Q, Wang Q, Shen D, Zhang Y, Tian G, Aisa H, Wei D, Jiang Y, Xiao G, Jiang H, Zhang L, Yu X, Shen J, Zhang S, Xu H
To Be Published
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*cp*cp*up*ap*up*ap*ap*cp*up*up*ap*ap*up*cp*u)-3') (4 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Rna-directed rna polymerase (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Covid-19 rdrp complex bound to penciclovir (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- [(2r)-4-(2-azanyl-6-oxidanylidene-3h-purin-9-yl)-2-(hydroxymethyl)butyl] dihydrogen phosphate (333 Da, Ligand)
- Pyrophosphate 2- (175 Da, Ligand)
- Rna (5'-r(p*ap*gp*ap*up*up*ap*ap*gp*up*up*ap*u)-3') (3 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Structure of COVID-19 RNA-dependent RNA polymerase (extended conformation) bound to penciclovir
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 7dok
Q-score: 0.554
Yin W, Luan X, Li Z, Xie Y, Zhou Z, Liu J, Gao M, Wang X, Zhou F, Wang Q, Wang Q, Shen D, Zhang Y, Tian G, Aisa H, Wei D, Jiang Y, Xiao G, Jiang H, Zhang L, Yu X, Shen J, Zhang S, Xu H
To Be Published
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*cp*cp*cp*up*ap*up*ap*ap*cp*up*up*ap*ap*up*cp*up*cp*ap*cp*ap*up*ap*gp*c)-3') (7 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (107 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(p*gp*cp*up*ap*up*gp*up*gp*ap*gp*ap*up*up*ap*ap*gp*up*up*ap*u)-3') (6 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- [(2r)-4-(2-azanyl-6-oxidanylidene-3h-purin-9-yl)-2-(hydroxymethyl)butyl] dihydrogen phosphate (333 Da, Ligand)
- Covid-19 rdrp complex (exntended conformation) bound to penciclovir (Complex from Severe acute respiratory syndrome coronavirus 2)
- Pyrophosphate 2- (175 Da, Ligand)
Cryo-EM structure of human RNA Polymerase I in elongation state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7ob9
Q-score: 0.519
Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ Pubmed: 34887565 DOI: doi:10.1038/s41594-021-00693-4 ]
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Human)
- Dna non-template strand (13 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Human)
- Rna (9 kDa, RNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Human)
- Rna polymerase i (Complex)
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Human)
- Dna template strand (13 kDa, DNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Rna and dna (Complex from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa43 (37 kDa, Protein from Human)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Human)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Human)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Human)